4.7 Article

Construction, Expression, and Evaluation of the Naturally Acquired Humoral Immune Response against Plasmodium vivax RMC-1, a Multistage Chimeric Protein

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MDPI
DOI: 10.3390/ijms241411571

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chimeric protein; Plasmodium vivax; protein construction; humoral immune response; B-cell epitope prediction; molecular modelling

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In this study, a P. vivax recombinant modular chimeric protein (PvRMC-1) composed of important antigenic regions was designed and expressed. The antigenicity of PvRMC-1 was assessed in naturally exposed individuals, and it was recognized by IgG and IgM antibodies. The study also observed a predominant cytophilic response mediated by IgG1 and IgG3. IgM levels were inversely correlated with age and time of residence in endemic areas, while IgG and IgM reactivity indexes were positively correlated with each other and inversely correlated with the time of the last malaria episode.
The PvCelTOS, PvCyRPA, and Pvs25 proteins play important roles during the three stages of the P. vivax lifecycle. In this study, we designed and expressed a P. vivax recombinant modular chimeric protein (PvRMC-1) composed of the main antigenic regions of these vaccine candidates. After structure modelling by prediction, the chimeric protein was expressed, and the antigenicity was assessed by IgM and IgG (total and subclass) ELISA in 301 naturally exposed individuals from the Brazilian Amazon. The recombinant protein was recognized by IgG (54%) and IgM (40%) antibodies in the studied individuals, confirming the natural immunogenicity of the epitopes that composed PvRMC-1 as its maintenance in the chimeric structure. Among responders, a predominant cytophilic response mediated by IgG1 (70%) and IgG3 (69%) was observed. IgM levels were inversely correlated with age and time of residence in endemic areas (p < 0.01). By contrast, the IgG and IgM reactivity indexes were positively correlated with each other, and both were inversely correlated with the time of the last malaria episode. Conclusions: The study demonstrates that PvRMC-1 was successfully expressed and targeted by natural antibodies, providing important insights into the construction of a multistage chimeric recombinant protein and the use of naturally acquired antibodies to validate the construction.

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