期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 24, 期 21, 页码 -出版社
MDPI
DOI: 10.3390/ijms242115775
关键词
Ginkgo biloba; ZnO-NPs; mineral elements; gene expression; GbF3H gene
This study investigates the effects of different concentrations of ZnO-NPs on the growth and accumulation of flavonoids in Ginkgo biloba. The results show that 25 mg/L of ZnO-NPs enhances plant growth and flavonoid content, while higher concentrations have an inhibitory effect. The promotion of flavonoid accumulation is mainly attributed to the upregulation of flavonol structural genes.
Ginkgo biloba is a highly valuable medicinal plant known for its rich secondary metabolites, including flavonoids. Zinc oxide nanoparticles (ZnO-NPs) can be used as nanofertilizers and nano-growth regulators to promote plant growth and development. However, little is known about the effects of ZnO-NPs on flavonoids in G. biloba. In this study, G. biloba was treated with different concentrations of ZnO-NPs (25, 50, 100 mg/L), and it was found that 25 mg/L of ZnO-NPs enhanced G. biloba fresh weight, dry weight, zinc content, and flavonoids, while 50 and 100 mg/L had an inhibitory effect on plant growth. Furthermore, quantitative reverse transcription (qRT)-PCR revealed that the increased total flavonoids and flavonols were mainly due to the promotion of the expression of flavonol structural genes such as GbF3H, GbF3'H, and GbFLS. Additionally, when the GbF3H gene was overexpressed in tobacco and G. biloba calli, an increase in total flavonoid content was observed. These findings indicate that 25 mg/L of ZnO-NPs play a crucial role in G. biloba growth and the accumulation of flavonoids, which can potentially promote the yield and quality of G. biloba in production.
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