期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 24, 期 14, 页码 -出版社
MDPI
DOI: 10.3390/ijms241411690
关键词
oligonucleotide insecticides; chrysanthemum aphid; plant protection; antisense technologies; gene expression; nuclease activity; eco-friendly insecticides
This article presents the results of using oligonucleotide insecticides to control the widespread pest Macrosiphoniella sanborni that damages Chrysanthemum morifolium. The study shows that the oligonucleotide insecticide is effective against wingless aphids, but has no effect on other aphids. This indicates the selective nature of oligonucleotide insecticides.
Macrosiphoniella sanborni is a widespread pest of Chrysanthemum morifolium that causes significant damage to world floriculture. Chemical insecticides and biological methods of control have a number of disadvantages that can be improved by using oligonucleotide insecticides. In this article, we present, for the first time, the results of using oligonucleotide insecticides, for which the target sequence is an internal transcribed spacer (ITS) in a polycistronic rRNA transcript. The mortality of wingless aphid individuals after a Macsan-11 treatment was recorded at a level of 67.15 & PLUSMN; 3.32% 7 days after a single treatment with a solution at a concentration of 100 ng/& mu;L and 97.38 & PLUSMN; 2.49% 7 days after a double treatment with a solution of the same concentration and a daily interval. The contact use of the control oligonucleotide (ACTG)(2)ACT-11. as well as the oligonucleotide insecticides Macsan-11(3 & PRIME;) and Macsan-11(5 & PRIME;) was not accompanied by insect mortality. Given the high variability in the internal transcribed spacer, which has proven to be a promising target for the action of oligonucleotide insecticides, it is possible to create selective preparations. This study showed the prospects of ribosomal insect pest genes as targets for the action of olinscides, and also demonstrated the high specificity of such insecticidal agents.
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