Carbamoylated Erythropoietin-Induced Cerebral Blood Perfusion and Vascular Gene Regulation

Cerebral hypoperfusion is associated with enhanced cognitive decline and increased risk of neuropsychiatric disorders. Erythropoietin (EPO) is a neurotrophic factor known to improve cognitive function in preclinical and clinical studies of neurodegenerative and psychiatric disorders. However, the clinical application of EPO is limited due to its erythropoietic activity that can adversely elevate hematocrit in non-anemic populations. Carbamoylated erythropoietin (CEPO), a chemically engineered non-erythropoietic derivative of EPO, does not alter hematocrit and maintains neurotrophic and behavioral effects comparable to EPO. Our study aimed to investigate the role of CEPO in cerebral hemodynamics. Magnetic resonance imaging (MRI) analysis indicated increased blood perfusion in the hippocampal and striatal region without altering tight junction integrity. In vitro and in vivo analyses indicated that hippocampal neurotransmission was unaltered and increased cerebral perfusion was likely due to EDRF, CGRP, and NOS-mediated vasodilation. In vitro analysis using human umbilical vein endothelial cells (HUVEC) and hippocampal vascular gene expression analysis showed CEPO to be a non-angiogenic agent which regulates the MEOX2 gene expression. The results from our study demonstrate a novel role of CEPO in modulating cerebral vasodilation and blood perfusion.

Automated summary

Cerebral hypoperfusion has negative effects on cognitive function and increases the risk of neuropsychiatric disorders. Erythropoietin (EPO) has been shown to improve cognitive function, but its use is limited due to its erythropoietic activity. However, carbamoylated erythropoietin (CEPO), a non-erythropoietic derivative of EPO, has similar neurotrophic and behavioral effects without affecting hematocrit levels. Our study reveals that CEPO can increase blood perfusion through neurotransmission and vasodilation, without affecting tight junction integrity.