期刊
CHEMICAL COMMUNICATIONS
卷 51, 期 36, 页码 7689-7692出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5cc01330e
关键词
-
资金
- Major State Basic Research Development Program of China (973 Program) [2012CB822102]
- National High Technology Research and Development Program of China [2012AA021504]
- National Natural Science Foundation of China [21172135, 21372145]
- Development Fund for Collaborative Innovation Center of Glycoscience of Shandong University
- NIH [R01HD065122]
Lacto-N-neotetraose and its sialyl and fucosyl derivatives including Lewis x (Le(x)) pentasaccharide, sialyl Lewis x (sLe(x)) hexasaccharide and internally sialylated derivatives were enzymatically synthesized from readily available lactoside, commercially available uridine 5'-diphosphate-glucose (UDP-Glc) and the corresponding mono-saccharides using a highly efficient sequential one-pot multienzyme (OPME) strategy. The OPME strategy which combines bacterial glycosyltransferases and sugar nucleotide generation enzymes provides easy access to the biologically important complex oligosaccharides at preparative scale. Moreover, the same OPME strategy can be used for the regioselective introduction of sialic acid to the internal galactose unit of LNnT in a designed glycosylation route by simply changing the glycosylation sequence.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据