期刊
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
卷 490, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.ijms.2023.117084
关键词
Orbitrap-IRMS; Isotope geochemistry; Stable isotopes; Clumped isotopes
Orbitrap-IRMS has been successfully applied to high-precision isotope ratio measurements of various compounds, but certain ratios require long experimental times to achieve desired precision. This study characterizes the performance of Orbitrap-IRMS for long duration measurements and explores the challenges of inter-measurement stability. Results show high precision for most ratios but limited accuracy due to inter-measurement drift. The study demonstrates the viability of Orbitrap-IRMS for extended measurements of challenging properties.
Orbitrap isotope ratio mass spectrometry (Orbitrap-IRMS) has recently been applied to high-precision, naturalabundance isotope ratio measurements of a diverse range of compounds, including amino acids, oxyanions, fatty acids, and metals. These measurements can characterize many isotope ratios simultaneously at high (R-.11.0%& sigma;) precision. In a successful experiment, observed precision will track the shot-noise limit and be limited by experimental time. Some isotope ratios, for example those involving 17O in organic compounds or multiplysubstituted ('clumped') isotopologues, require experimental times of hours to tens of hours to achieve desired precision, while current sample introduction techniques focus on observations on the order of seconds to tens of minutes. In this study, we characterize Orbitrap-IRMS performance for three long duration measurements (individual acquisitions & GE;1 h and as long as 24 h) using an automated reservoir injection system coupled to a Q Exactive HF Orbitrap with an electrospray ionization (ESI) source. First, we characterize long-term intra-measurement stability through a 24-h long measurement of acetone. We report the following isotope ratios and precisions (as acquisition errors, errors on the observed ratio within this measurement (& sigma;AE)): 13C/12C (& sigma;AE = 0.07%& sigma;), 17O/16O (& sigma;AE = 1.1%& sigma;), 18O/16O (& sigma;AE = 0.3%& sigma;), and 13C13C/12C (& sigma;AE = 0.65%& sigma;). The & sigma;AE of each tracks the shot noise limit throughout and is limited by the challenging conditions (high resolution and low numbers of ions per scan) required for 17O/16O measurement in the presence of 13C via Orbitrap. Second, we characterize inter-measurement stability via a sequence of seven 75-min analyses of perchlorate. We observe the following ratios and acquisition errors: 37Cl/35Cl (& sigma;AE = 0.09%& sigma;); 17O/16O (& sigma;AE = 1.6%& sigma;); 18O/16O (& sigma;AE = 0.7%& sigma;), 37Cl17O/35Cl16O (& sigma;AE = 2.7%& sigma;), and 37Cl18O/35Cl16O (& sigma;AE = 1.2%& sigma;). However, we find that inter-measurement drift between acquisitions limits our accuracy and precision for standardized measurements (i.e., error on reported & delta; values) to R-.11%& sigma; for the 37Cl/35Cl measurement. Hence, the benefits of low & sigma;AE may not be fully realized. Third, we demonstrate accuracy via sample/standard comparisons of a methionine sample with 13C enrichment of R-.120%& sigma; relative to a known standard. Using a sequence of seven 60-min analyses, we recover the following isotope ratios and standardized precisions (i.e., error on reported & delta; values, denoted propagated acquisition errors, & sigma;PAE): 33S/32S (& sigma;PAE = 1.0%& sigma;), 34S/32S (& sigma;PAE = 0.7%& sigma;), 15N/14N (& sigma;PAE = 2.1%& sigma;), 2H/1H (& sigma;PAE = 3.2%& sigma;),13C/12C (& sigma;PAE = 0.4%& sigma;), 18O/16O (& sigma;PAE = 1.6%& sigma;), & 13C13C/12C (& sigma;PAE = 2.8%& sigma;) with confirmation of accurate results for the known 13C/12C and 13C13C/12C enrichments. Together, our results demonstrate the viability of Orbitrap-IRMS for long duration measurements of diverse sample types via an automated reservoir injection system. Intermeasurement stability remains a challenge; we expect our methods to be most applicable to extended measurements of hard-to-observe properties, such as 17O in organics and clumped isotopologues.
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