期刊
MICROCHIMICA ACTA
卷 183, 期 3, 页码 1099-1106出版社
SPRINGER WIEN
DOI: 10.1007/s00604-015-1695-1
关键词
Multiplex assay; Dendritic polymerase; Gold nanoparticles; Magnetic nanoparticles; Metal marker; Square wave stripping voltammetry
资金
- National Natural Science Foundation of China [31070866]
- Natural Science Foundation of Zhejiang [Y15B050002, 2014A610184]
- Natural Science Foundation of Ningbo [Y15B050002, 2014A610184]
- K.C. Wong Magna Fund in Ningbo University
- State Administration of Grain [201313010]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
The article describes a multiplex aptamer based voltammetric assay for the food pollutants chloramphenicol (CAP) and polychlorinated biphenyl-72 (PCB72). The method is based on the displacement of a nanotracer (an aptamer-labeled magnetic dendritic probe) by the analytes which cause the release of the corresponding encoded nanotracers. Thiolated aptamers of CAP and PCB72 were first self-assembled on magnetic gold nanoparticles (Fe3O4@Au) as capture probes to concentrate the target analytes CAP and PCB72, respectively. Next, their hybridized complementary strands (cDNA1 or cDNA2) and a dendritic polymerase were linked to quantum dots (CdS or PbS) to form the respective nanotracers. Finally, encoded composite magnetic probes were obtained through hybridization reaction between aptamers and cDNAs. Once CAP and PCB72 are recognized by the composite probes, the nanotracers are released into the supernatant where they can be detected simultaneously by voltammetry of the metal ion markers Cd(II) and Pb(II) at -0.81 and -0.57 V (vs. Ag/AgCl), respectively. The concentration measured for Cd(II) and Pb(II) are directly proportional to the concentrations of CAP and PCB72, respectively, in the range from 0.001 to 100 ng mL(-1). The detection limits for CAP and PCB72 are 0.33 and 0.35 pg mL(-1), respectively (at an S/N ratio of 3), which is 2 to 3 orders of magnitude lower than the routinely performed and commercially available ELISA. The method is perceived to possess large potential in that it may be applied to other antibiotics by using the appropriate aptamers.
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