Comparative genomics reveals key molecular targets for mutant Pediococcus pentosaceus C23221 producing pediocin

Listeria monocytogenes is a common microorganism that causes food spoilage. Pediocins are some biologically active peptides or proteins encoded by ribosomes, which have a strong antimicrobial activity against L. monocytogenes. In this study, the antimicrobial activity of previously isolated P. pentosaceus C-2-1 was enhanced by ultraviolet (UV) mutagenesis. A positive mutant strain P. pentosaceus C23221 was obtained after 8 rounds of UV irradiation with increased antimicrobial activity of 1448 IU/mL, which was 8.47 folds higher than that of wild-type C-2-1. The genome of strain C23221 and wild-type C-2-1 was compared with identify the key genes for higher activity. The genome of the mutant strain C23221 consists of a chromosome of 1,742,268 bp, with 2052 protein-coding genes, 4 rRNA operons, and 47 tRNA genes, which is 79,769 bp less than the original strain. Compared with strain C-2-1, a total of 19 deduced proteins involved in 47 genes are unique to C23221 analyzed by GO database; the specific ped gene related to bacteriocin biosynthesis were detected using anti -SMASH in mutant C23221, indicating mutant C23221 produced a new bacteriocin under mutagenesis conditions. This study provides genetic basis for further constituting a rational strategy to genetically engineer wild-type C-2-1 into an overproducer.

Automated summary

In this study, the antimicrobial activity of P. pentosaceus C-2-1 was enhanced by UV mutagenesis, resulting in the production of a mutant strain, C23221, with significantly higher antimicrobial activity. A comparison of the genomes of C23221 and the wild-type strain revealed unique genes and proteins related to bacteriocin biosynthesis in the mutant strain. This study provides a genetic basis for future genetic engineering strategies to increase antimicrobial production in P. pentosaceus C-2-1.