期刊
MICROCHIMICA ACTA
卷 183, 期 4, 页码 1469-1477出版社
SPRINGER WIEN
DOI: 10.1007/s00604-016-1790-y
关键词
Nanomaterial; MIP; Vibrating magnetometry; Saturation magnetization; Transmission electron microscopy; Core-shell particles; UHPLC-MS/MS; FTIR; Food analysis
资金
- National Natural Science Foundation of China [21505026, 21275057]
- Medical Science and Technology Project of Guangzhou [20141 A010055]
This article describes a method for determination of four aflatoxins by a combination of ultra-high performance liquid chromatography and mass spectrometry (UHPLC-MS/MS). Magnetic dummy molecularly imprinted polymers (md-MIPs) are used for fairly selective preconcentration. The preparation of the md-MIP involves (a) coating of magnetic nanoparticles (with a typical diameter of 200 nm) with a shell of silica, (b) modifying the surface with methacryloxy groups, and (c) creating a MIP on the surface by graft imprinting in aqueous solutions using 5,7-dimethoxycoumarin as the dummy template, and methacrylic acid and 4-vinylpyridine as the functional monomers. After elution of the template, the md-MIPs can be used for fairly selective extraction of the aflatoxins B1, B2, G1 and G2. Following magnetic separation of the particles, the aflatoxins were eluted and quantified by UHPLC-MS/MS. The limit of detection is 0.05 mu g center dot kg(-1) for aflatoxins B2 and G2, and 0.1 mu g center dot kg(-1) for aflatoxins B1 and G1. False-positive samples were identified by UHPLC-MS/MS in the positive ionization mode with multiple reaction monitoring.
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