期刊
MICROCHIMICA ACTA
卷 183, 期 6, 页码 1839-1846出版社
SPRINGER WIEN
DOI: 10.1007/s00604-016-1814-7
关键词
HPLC; DAD; Permeation selectivity; Ofloxacin; Norfloxacin; Enrofloxacin; Prulifloxacin; Manure
资金
- National High Technology Research and Development Program of China [2012AA06A304]
The determination of antibiotics in biological samples is compromised by their interaction with proteins. It is showed here that the water resistant zeolite imidazolate framework 67 (ZIF-67) represents a useful sorbent for solid-phase extraction (SPE) of fluoroquinolones (FQs) from biological samples. ZIF-67 is unique in possessing permanent nanoscale porosity and a high surface area. As a result, it efficiently excludes proteins from the inner network but can well adsorb small organic molecules. Its extraction capacity for FQs exceeds that of isostructural ZIF-8, probably due to high affinity for Co(II) ions in ZIF-67. The findings were exploited to design a method for the analysis of FQs, which were extracted from bio-samples by ZIF-67 via SPE. Following elution of the absorbed FQs with NaOH/methanol, they were quantified by HPLC with diode array detection. The assay has a wide linear range that extends from 3.9 to 4000 mu ga <...kgaEuro3/4(1), with detection limits between 1.2 and 2.9 mu ga <...kgaEuro3/4(1). The method was successfully applied to the determination of FQs in manure.
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