期刊
MICROCHIMICA ACTA
卷 183, 期 12, 页码 3093-3099出版社
SPRINGER WIEN
DOI: 10.1007/s00604-016-1951-z
关键词
Nanobiosensor; Mycotoxin; Core-shell nanoparticles; Capping; Magnetic nanoparticles; CdTe; Transmission electron microscopy; Dynamic light scattering; Food contamination
资金
- Nanozino RD Company
We report on a highly sensitive competitive immunoassay for the mycotoxin Ochratoxin (OTA) using magnetic silica nanoparticles (NPs) fluorescently labeled with rhodamine 123 (Rho123) as signal intensifier. The method is based on the measurement of fluorescence resonance energy transfer (FRET) that occurs from CdTe quantum dots covered with anti-OTA antibody to the dye Rho123 on the surface of the NPs. The immunoreaction between anti-OTA antibody and OTA brings the fluorophore (acting as the acceptor) in close proximity of the QDs (acting as the donor), and this causes FRET to occur upon photo-excitation of the QDs. The size and polydispersity of the silica coated magnetic NPs was studied via TEM. The method has a detection limit of 0.8 pg of OTA per mL. It was applied to the determination of OTA in spiked human serum. A linear relationship is found between the increase in the fluorescence intensity of Rho 123 at 580 nm and the concentration of OTA in spiked samples over the 8 to 48 pgai...mL(-1) concentration range. This highly sensitive homogeneous competitive detection scheme is simple, rapid and efficient. It does not require multiple separation steps and excessive washing.
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