期刊
MICROCHIMICA ACTA
卷 184, 期 2, 页码 547-555出版社
SPRINGER WIEN
DOI: 10.1007/s00604-016-2046-6
关键词
Magnetic mesoporous carbon; Ferric oxide; Zirconia; Nanoparticles; Hydrophilic carbon material; Metal oxide affinity chromatography; Phosphopeptide; Enrichment; Human serum; MALDI-TOF MS
资金
- State Key Program of National Natural Science of China [21235005]
- National Key Scientific Instrument and Equipment Development Project [2012YQ120044]
- National Natural Science Foundation of China [21475044]
- model Project of the Research and Application of the Common Key Technology about Chemical Reagent of Basis Scientific Research [2015BAK44B00]
- foundation of Shanghai Research Institute of Criminal Science and Technology
Magnetic mesoporous carbon composites incorporating hydrophilic metallic nanoparticles were synthesized from resol, ZrO(NO3)(2), ferric acetylacetonate, and triblock copolymer F127. The method involves a multi-component co-assembly strategy associated with direct carbonization. The resulting carbon material is shown to be useful as a metal oxide affinity chromatography (MOAC) material for enrichment of phosphopeptides owing to its large mesoporous (4.8 nm) surface area (442 m(2) g(-1)), large pore volume (0.37 cm(3) g(-1)) and excellent hydrophilicity. The metallic iron and ferric oxide particles modified on the mesoporous carbon exert a magnetic force and, in combination with the metallic zirconia, is a viable MOAC material for enrichment of low-abundance phosphopeptides. Because of metal chelation between metallic nanoparticles and the phosphate groups of phosphopeptides, the zirconia/magnetic mesoporous carbon displays high selectivity even at a phosphopeptide/nonphosphopeptide molar ratio of 1:500. As little as 1.5 fmol of phosphopeptides become detectable. The MOAC was successfully applied to the identification by MALDI-TOF MS of phosphopeptides in human serum and nonfat milk.
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