Development and applications of enzymatic modified starch with high water solubility providing a continuous supply of glucose

Amylopectin clusters (APCs) are produced by cyclodextrin glucanotransferase (EC 2.4.1.19). Their solubility rate in aqueous solution was found to be 16.7 %. The weight-average molecular weight of APCs is similar to 10(5) Da, as determined by multiangle laser light scattering analysis. Side chain length analysis indicated that the relative proportions of side chains with a degree of polymerization in the ranges of 2-8 and 25-50 decreased and increased, respectively, during preparation of APCs. In the exercise experiment, the blood glucose level of rats was higher in the APC-treated group than in the groups treated with commercial carbohydrate supplement (CCD) and glucose. In the forced swimming test, the swimming time in the APC and CCD groups increased by 22.6 % and 31.1 %, respectively, compared with the glucose administration group. The insulin levels were also similar between the APC and CCD groups. However, the glycogen levels in the liver and muscles of mice were significantly higher in the APC group than control group. These results suggest that APCs could potentially enhance endurance when added to sports drinks.

Automated summary

Amylopectin clusters (APCs) with a solubility rate of 16.7% were produced using cyclodextrin glucanotransferase (EC 2.4.1.19). Their weight-average molecular weight was determined to be around 10(5) Da. During the preparation of APCs, the proportions of side chains with certain degree of polymerization changed. In exercise experiments, rats treated with APCs had higher blood glucose levels and increased endurance compared to the glucose administration group, indicating the potential of APCs in enhancing endurance when added to sports drinks.