Structural characterization and anti-oxidation activity of pectic polysaccharides from Swertia mileensis

In this study, we isolated the pectic polysaccharide WSMP-A2b (37 kDa) from the stems and leaves of Swertia mileensis, and we investigated its compositional/structural features and antioxidant activity. FT-IR, NMR, monosaccharide composition, enzymatic hydrolysis and methylation analyses indicated that WSMP-A2b is composed of rhamnogalacturonan I (RG-I), rhamnogalacturonan II (RG-II) and homogalacturonan (HG) domains with mass ratios of 2.1:1.0:2.2. The RG-I domain is primarily substituted with & alpha;-L-1,5-arabinan and type II arabinogalactan (AG-II) side chains, as well as minor contributions of & beta;-D-1,4-galactan and/or type I arabino-galactan (AG-I) side chains. The HG domain was released in the form of un-esterified and partly methyl-esterified and/or acetyl-esterified oligogalacturonides with a 1 to 7 degree of polymerization after endo-polygalacturonase degradation. WSMP-A2b showed stronger antioxidant activity in vitro, in part this might due to the presence of galacturonic acid (GalA). In addition, WSMP-A2b exerted a protective effect on tert-butyl hydroperoxide (tBHP)-induced oxidative stress in INS-1 cells by reducing reactive oxygen species (ROS) production and increasing the glutathione/oxidized glutathione (GSH/GSSG) ratio. Our results provide crucial structural information on this pectic polysaccharide from Swertia mileensis, thus prompting further investigation into its structure-activity relationship.

Automated summary

In this study, we isolated and characterized a pectic polysaccharide, WSMP-A2b, from Swertia mileensis. WSMP-A2b is mainly composed of RG-I, RG-II, and HG domains, with a mass ratio of 2.1:1.0:2.2. WSMP-A2b exhibited strong antioxidant activity, potentially attributed to its high galacturonic acid content. Moreover, WSMP-A2b showed protective effects against oxidative stress in INS-1 cells by reducing ROS production and increasing the GSH/GSSG ratio.