期刊
MICROCHIMICA ACTA
卷 184, 期 2, 页码 515-523出版社
SPRINGER WIEN
DOI: 10.1007/s00604-016-2045-7
关键词
Fluorescent bioassay; Nanoprobes; Glucose detection; Glucose oxidase; Enzymatically generated H2O2; Glucuronic acid; Mn(II) ions
资金
- National Natural Science Foundation of China [21305119, 21375114]
- Nanhu Scholars Program for Young Scholars of XYNU
- Plan For Scientific Innovation Talent of Henan Province
The authors describe a fluorometric glucose assay that is based on the use of MnO2 nanosheets and copper nanoclusters (CuNCs) acting as nanoprobes. The CuNCs were synthesized by using bovine serum albumin as a template by chemical reduction of copper(II) sulfate. On addition of MnO2 nanosheets to a colloidal solution of CuNCs, the fluorescence of CuNCs (measured at excitation/emission wavelengths of 335/410 nm) is quenched. However, in the presence of enzymatically generated H2O2, the MnO2 nanosheets are reduced to form Mn(II) ions. As a result, fluorescence intensity recovers. The glucose assay is based on the enzymatic conversion of glucose by glucose oxidase to generate H2O2 and glucuronic acid. The calibration plot is linear in the 1 mu M to 200 mu M glucose concentration range, and the detection limit is 100 nM. The method was successfully applied to the determination of glucose in spiked human serum samples.
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