期刊
MICROCHIMICA ACTA
卷 184, 期 1, 页码 65-72出版社
SPRINGER WIEN
DOI: 10.1007/s00604-016-1981-6
关键词
Metal nanoparticles; Enzyme mimic; Electron paramagnetic resonance; Lineweaver-Burk diagram; Michaelies-Menten kinetics
资金
- National Natural Science Foundation of China [31371767]
- National S&T support program of China [2015BAD17B02]
- Natural Science Foundation of Jiangsu Province [BK20141108]
The authors report that the peroxidase-like activity of Au@Pt core-shell nanohybrids (Au@PtNHs) is selectively inhibited by cysteine. This finding has led to a highly sensitive colorimetric assay for cysteine that is based on the nanohybrid-catalyzed oxidation of TMB by H2O2 to form a blue product. The method has a detection limit of 5.0 nM and a linear range from 10 nM to 20 mu M. The assay is highly selective over other amino acids. It was successfully applied to the determination of cysteine in an injection containing a mixture of amino acids.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据