4.7 Article

Glutamine protects intestinal immunity through microbial metabolites rather than microbiota

期刊

INTERNATIONAL IMMUNOPHARMACOLOGY
卷 124, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.intimp.2023.110832

关键词

Glutamine; Metabolomics profiling; Intestinal microbiota; Intestinal immunity; Mice

向作者/读者索取更多资源

This study investigated the effects of glutamine supplementation on the intestinal microbiota and microbial metabolites. The results showed no significant changes in the microbiota, but differences were observed in the metabolites. Metabolomics analysis revealed alterations in various metabolic pathways and differential metabolites, which are beneficial for intestinal development and immunity. Overall, glutamine supplementation may maintain intestinal homeostasis and improve intestinal immunity through the modulation of intestinal microbial metabolites.
Glutamine has anti-inflammatory properties as well as the ability to maintain the integrity of the intestinal barrier. In our previous study, we found that 1.0% glutamine promoted SIgA (secretory immunoglobulin A) synthesis in the gut via both T cell-dependent and non-dependent processes, as well as via the intestinal microbiota. The purpose of this study was to investigate whether the intestinal microbiota or microbial metabolites regulate SIgA synthesis. In the mouse model, supplementation with 1.0% glutamine had no significant effect on the intestinal microbiota, but KEGG function prediction showed the difference on microbiota metabolites. Therefore, in this study, untargeted metabolomics techniques were used to detect and analyze the metabolic changes of glutamine in intestinal luminal contents. Metabolomics showed that in the positive ion (POS) mode, a total of 1446 metabolic differentials (VIP = 1, P < 0.05, FC = 2 or FC = 0.5) were annotated in samples treated with glutamine-supplemented group compared to control group, of which 922 were up-regulated and 524 down-regulated. In the negative ion (NEG) mode, 370 differential metabolites (VIP = 1, P < 0.05, FC = 2 or FC = 0.5) were screened, of which 220 were up-regulated and 150 down-regulated. These differential metabolites mainly include bile secretion synthesis, ABC transporters, diterpenoids and other secondary metabolites. KEGG analysis showed that propionic acid metabolism, TCA cycle, endoplasmic reticulum protein processing, nitrogen metabolism and other metabolic pathways were active. The above metabolic pathways and differential metabolites have positive effects on intestinal development and intestinal immunity, and combined with our previous studies, we conclude that glutamine supplementation can may maintain intestinal homeostasis and improving intestinal immunity through intestinal microbial metabolites.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据