The appearance of cytoplasmic cytochrome C precedes apoptosis during Drosophila salivary gland degradation

Apoptosis is an important process for organism development that functions to eliminate cell damage, maintain homeostasis, and remove obsolete tissues during morphogenesis. In mammals, apoptosis is accompanied by the release of cytochrome C (Cyt-c) from mitochondria to the cytoplasm. However, whether this process is conserved in the fruit fly, Drosophila melanogaster, remains controversial. In this study, we discovered that during the degradation of Drosophila salivary gland, the transcription of mitochondria apoptosis factors (MAPFs), Cyt-c, and death-associated APAF1-related killer (Dark) encoding genes are all upregulated antecedent to initiator and effector caspases encoding genes. The proteins Cyt-c and the active caspase 3 appear gradually in the cytoplasm during salivary gland degradation. Meanwhile, the Cyt-c protein colocates with mito-GFP, the marker indicating cytoplasmic mitochondria, and the change in mitochondrial membrane potential coincides with the appearance of Cyt-c in the cytoplasm. Moreover, impeding or promoting 20E-induced transcription factor E93 suppresses or enhances the staining of Cyt-c and the active caspase 3 in the cytoplasm of salivary gland, and accordingly decreases or increases the mitochondrial membrane potential, respectively. Our research provides evidence that cytoplasmic Cyt-c appears before apoptosis during Drosophila salivary gland degradation, shedding light on partial conserved mechanism in apoptosis between insects and mammals.

Automated summary

Apoptosis is a crucial process for organism development, and our study reveals the presence of cytoplasmic cytochrome C during Drosophila salivary gland degradation, indicating a partially conserved mechanism in apoptosis between insects and mammals. The upregulation of mitochondria apoptosis factors, cytochrome C, and death-associated APAF1-related killer encoding genes, as well as the colocation of cytochrome C with cytoplasmic mitochondria marker mito-GFP, suggest the involvement of mitochondrial pathway in apoptosis. Furthermore, the inhibition or promotion of the transcription factor E93 affects the staining of cytochrome C and active caspase 3, and the mitochondrial membrane potential.