期刊
MICROBIOLOGICAL RESEARCH
卷 191, 期 -, 页码 19-31出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.micres.2016.05.010
关键词
2,4-Di-tert-butyl-phenol; Biofilm; GAS; Hydrophobicity; Hyaluronic acid
类别
资金
- Department of Biotechnology, Government of India [BT/BI/25/012/2012 (BIF)]
- University Grants Commission, New Delhi through SAP-DRS1 [F.3-28/2011(SAP-II)]
- Department of Science and Technology, Government of India through PURSE [SR/S9Z-23/2010/42(G)]
- FIST [SR-FST/LSI-087/2008]
- Council of Scientific and Industrial Research (CSIR), New Delhi [09/688(0017)/2011-EMR-I]
Biofilm formation of Group A Streptococcus (GAS) is recognized as an important virulent determinant. The present study reports the antibiofilm potential of seaweed (Gracilaria gracilis) surface associated Bacillus subtilis against GAS. Purification revealed 2,4-Di-tert-butyl-phenol (DTBP) as the active principle. DTBP exhibited a dose dependent antibiofilm activity against GAS (SF370 & six different clinical M serotypes). Microscopic analysis, revealed changes in cell surface architecture and reduced thickness upon DTBP treatment. Results of extracellular polymeric substance quantification, microbial adhesion to hydrocarbon assay and fourier transform infrared spectroscopic analysis suggested that DTBP probably interferes with the initial adhesion stage of biofilm formation cascade. Reduction in hyaluronic acid synthesis goes in unison with blood survival assay wherein, increased susceptibility to phagocytosis was observed. In vivo studies using Caenorhabditis elegans manifested the reduction in adherence and virulence, which prompts further investigation of the potential of DTBP for the treatment of GAS infections. (C) 2016 Elsevier GmbH. All rights reserved.
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