期刊
MICROBIOLOGICAL RESEARCH
卷 186, 期 -, 页码 139-145出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.micres.2016.03.008
关键词
Polygonum cuspidatum; Emodin; Haemophilus parasuis; Antibacterial activity
类别
资金
- National Science &Technology Program in Rural Areas during the 12th Five Year Plan Period [2011BAD34B03-4]
- Sichuan Youth Science and Technology Innovation Research Team for waterfowl disease prevention and control [2013TD0015]
- International cooperation projects of Sichuan Province [2014HH0058, 2013HH0042]
- National Natural Science Foundation of China [31372477]
Haemophilus parasuis is the causative agent of Glasser's disease, which leads to serious economic loss to the swine industry. Although antibiotics are widely used to control infections, outbreaks of this disease repeatedly happen. In this study, emodin from Polygonum cuspidatum showed potent inhibitory effect against H. parasuis. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values of emodin were 32 and 64 mu g/mL, respectively. The antibacterial kinetic curves indicated the antibacterial activity of emodin was in a concentration-dependent manner. Cell membrane permeability and flow cytometry assays proved that emodin could destroy cell membrane integrity and increase membrane permeability, and fluorescence spectra assay indicated emodin has influenced conformation of membrane protein. Under transmission electron microscopy, serious lesions of H. parasuis exposed to emodin (64 mu g/mL) were found, including irregular cell shape, plasmolysis, ruptured cell wall and membrane and cytoplasmic vacuolation. These results suggested that emodin could be used as candidate for treating Glasser's disease. (C) 2016 Elsevier GmbH. All rights reserved.
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