期刊
MICROBIAL PATHOGENESIS
卷 100, 期 -, 页码 56-61出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2016.09.007
关键词
Avian pathogenic E. coli (APEC); Chaperone-usher fimbriae; yfcO; Adhesion; Virulence
资金
- National Key R D Program [2016YFD0500804]
- National Natural Science Foundation of China [31672576]
- Science and technology basic work of science and technology [2012FY111000]
- Fund of Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Fundamental Research Funds for the Central Universities [KJQN201525, KYZ201326]
Chaperone-usher (CU) fimbriae, which are adhesive surface organelles found in many Gram-negative bacteria, mediate tissue tropism through the interaction of fimbrial adhesins with specific receptors expressed on the host cell surface. A CU fimbrial gene yfcO, was identified in avian pathogenic E. coli (APEC) strain DE205B via gene functional analysis. In this study, yfcO was found in 13.41% (11/82) of E. coli strains, including phylogenetic groups A, Bl, B2 and D, with the highest percentage in group B2. The expression of yfcO in biofilm forming bacteria was significantly higher (P < 0.05) than that in the planktonic bacteria. A yfcO deletion mutant was constructed, and adherence to DF-1 chicken embryo fibroblast cells was analyzed in vitro. Compared to the wild-type (WT), adherence of the mutant to DF-1 cells was significantly decreased (P < 0.01). The mutant bacterial loads in the heart, brain and liver were significantly lower (P < 0.05) than those of the WT strain. Resistance of the mutant to acidic (acetic, pH 4.0, 20 min) and high osmolarity (2.5 M NaCl, 1 h) stress conditions decreased by 51.28% (P < 0.001) and 80.34% (P < 0.01), respectively. These results suggest that yfcO contributes to APEC virulence through bacterial adherence to host tissues. (C) 2016 Elsevier Ltd. All rights reserved.
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