期刊
METHODS
卷 100, 期 -, 页码 3-15出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2016.02.017
关键词
Electron microscopy
资金
- Wellcome Trust [096685/Z/11/Z]
- Wellcome Trust [096685/Z/11/Z] Funding Source: Wellcome Trust
- MRC [G1000567] Funding Source: UKRI
- Medical Research Council [G1000567] Funding Source: researchfish
Transmission electron microscopy (EM) is a versatile technique that can be used to image biological specimens ranging from intact eukaryotic cells to individual proteins >150 kDa. There are several strategies for preparing samples for imaging by EM, including negative staining and cryogenic freezing. In the last few years, cryo-EM has undergone a 'resolution revolution', owing to both advances in imaging hardware, image processing software, and improvements in sample preparation, leading to growing number of researchers using cryo-EM as a research tool. However, cryo-EM is still a rapidly growing field, with unique challenges. Here, we summarise considerations for imaging of a range of specimens from macro molecular complexes to cells using EM. (C) 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.orgilicensesiby/4.0/).
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