In vitro selection and amplification protocols for isolation of aptameric sensors for small molecules

We recently optimized a procedure that directly yields aptameric sensors for small molecules in so-called structure-switching format. The protocol has a high success rate, short time, and is sufficiently simple to be readily implemented in a non-specialist laboratory. We provide a stepwise guide to this selection protocol. (C) 2016 Elsevier Inc. All rights reserved.