期刊
METHODS
卷 106, 期 -, 页码 58-65出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2016.04.032
关键词
Aptamer; Biosensor; SELEX; Small molecules; Structure-switching
资金
- National Science Foundation - United States [CBET-1033288, CBET-1026592]
- National Institutes of Health - United States [RGM104960]
- Division of Computing and Communication Foundations
- Direct For Computer & Info Scie & Enginr [1518715] Funding Source: National Science Foundation
We recently optimized a procedure that directly yields aptameric sensors for small molecules in so-called structure-switching format. The protocol has a high success rate, short time, and is sufficiently simple to be readily implemented in a non-specialist laboratory. We provide a stepwise guide to this selection protocol. (C) 2016 Elsevier Inc. All rights reserved.
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