Healthy live birth following embryo transfer of a blastocyst of tetrapronuclear (4PN) origin: a case report

During IVF treatments, normal fertilization is generally evidenced by the appearance of two pronuclei, one arising from the oocyte and the other from the male gamete. Embryos derived from zygotes with a pronuclei number other than two are assumed to possess a ploidy abnormality and their transfer is usually avoided owing to increased risk of implantation failure, miscarriage, and molar pregnancies. Nonetheless, the inclusion of genotyping data in preimplantation genetic testing has revealed that a normal diploid configuration is possible in embryos deriving from zygotes with an abnormal pronuclei number such as tripronuclear and one pronucleus. Here, we present a one-of-a-kind transfer of a tetrapronuclear-derived embryo that was discovered to be diploid and negative for other whole chromosome or segmental aneuploidies during preimplantation genetic testing using a targeted next-generation sequencing approach. The transfer resulted in the live birth of a healthy infant who is now 4 years old and has no apparent health or developmental impairments.

Automated summary

During IVF treatments, embryos with abnormal pronuclei number are usually not transferred due to increased risks. However, a unique case of transferring a tetrapronuclear-derived embryo resulted in the birth of a healthy infant using targeted next-generation sequencing for preimplantation genetic testing. The infant, now 4 years old, has no apparent health or developmental impairments.