期刊
HEARING RESEARCH
卷 434, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.heares.2023.108784
关键词
Presbycusis; JNK; Hair cell; Spiral ganglion; Stria vascularis
The study aimed to explore the spatio-temporal changes in JNK isoforms in the cochleae of mice with age-related hearing loss. Immunohistochemistry staining and western blotting were used to test the changes in JNK isoforms in an animal model and a senescent cell line. Results showed that all three JNK isoforms are present in the cochleae, and their expression patterns differ in different cell types. JNK3 is highly expressed in hair cells and increases with age-related hearing loss, suggesting its critical role in hair cell loss and spiral ganglion degeneration.
The c-Jun N-terminal kinase (JNK) pathway is a vital component of the mitogen-activated protein kinase cascade, which regulates cell death and survival. The present study aimed to explore the Spatio-temporal changes in all JNK isoforms in the cochleae of C57/BL6J mice with age-related hearing loss. Changes in the three isoforms of JNKs in the cochleae of an animal model with presbycusis and the senescent HEI-OC1 cell line were tested by immunohistochemistry staining and western blotting. Our results demonstrated that all three JNK isoforms are distributed in the cochleae, and the expression patterns of JNK1, JNK2, and JNK3 differed in hair cells, spiral ganglion neurons, and stria vascularis, with great significance in the cochleae of adult C57BL/6J mice. The levels of JNK1, JNK2, and JNK3 showed various spatio-temporal changes in the aging mice. In a senescent hair cell model, changes in JNK1, JNK2, and JNK3 expression levels were similar to those observed in the cochleae. Our study is the first to show that JNK3 is highly expressed in the hair cells of C57BL/6J mice and further increases in conjunction with age-related hearing loss, suggesting that it may play a more critical role than previously believed in hair cell loss and spiral ganglion degeneration.(c) 2023 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )
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