Molecular cloning of estrogen receptor and its function on vitellogenesis in pompano (Trachinotus ovatus)

Estrogen receptors (ERs) play a critical role in vitellogenesis (Vtgs). However, the contribution of each ER for the regulation of vtgs expression was not analyzed clearly in teleosts. In the present study, three ers isoforms (er alpha, erfi1, and erfi2) were cloned in pompano (Trachinotus ovatus). Real-time PCR and enzyme-linked immunosorbent assay (ELISA) was used to detect the effects of 17 beta-estradiol (E2) on ERs and Vtgs in the liver of pompano. In vivo injection experiments showed that E2 significantly increased the expressions of ers and vtgs. ER broad spectrum antagonist Fulvestrant significantly attenuated the E2- induced up-regulation of ers and vtgs in a dose-dependent manner. ER alpha antagonist Methyl-piperidino pyrazole (MPP) significantly attenuated the up-regulation of er alpha, erfi2, vtg-B and vtg-C, and promoted the expressions of erfi1 and vtg-A. ER beta antagonist Cyclofenil significantly inhibited the expressions of erfi1, erfi2, vtg-A and vtg-C, and promoted the expressions of er alpha and vtg-B. In addition, E2 significantly increased the protein level of Vtg, while Fulvestrant, MPP and Cyclofenil significantly inhibited the protein level of Vtg in a dose-dependent manner. Our results indicate that E2 may regulate the expression of each vtg with different subtypes of ERs, and shows a distinct compensatory expression effect on the regulation for ers and vtgs, which provides a theoretical basis for reproductive endocrinology study in pompano.

Automated summary

This study investigated the role of estrogen receptors (ERs) in vitellogenesis (Vtgs) regulation in pompano fish. The findings suggest that E2 may regulate the expression of different subtypes of vtg through ERs, displaying a compensatory expression effect on the regulation of ers and vtgs. This provides a theoretical basis for further research on reproductive endocrinology in pompano fish.