期刊
GENE
卷 881, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.gene.2023.147637
关键词
Genome size; Nuclear DNA content; Mulberry; Germplasm; Flow cytometry
This study investigates the variability in DNA content and genome size, chromosome number, ploidy and its relation with stomatal characteristics among 162 mulberry germplasm collection. It found that the majority of cultivated mulberries are diploids, with the 2C DNA content positively correlated with ploidy status and stomatal length. This study provides essential information for the mulberry breeding program.
The large size of the germplasm collection along with scanty information on their cytological and genome constitution have hindered well-planned breeding schemes in mulberry. To address the issue, a study was un-dertaken to investigate the variability in DNA content and genome size, chromosome number, ploidy and its relation with important stomatal characteristics among 162 mulberry germplasm collection. These germplasm comprise a core subset of 150 collections along with a representative collection of different mulberry species including the wild. Among the germplasm belonging to 16 species, we identified 122 diploids (2n = 28), 4 aneuploids (2n = 30), 13 triploids (2n = 42), 15 tetraploids (2n = 56), 7 hexaploids (2n = 84) and 1 dodeco-saploid (2n = 308) based on the chromosome count. Most of the cultivated mulberries are found to be diploids. The mean nuclear 2C DNA content estimated by Flow cytometry, varied from 0.723 & PLUSMN; 0.006 pg (M. australis, 2n = 2x) to 7.732 pg (M. nigra, 2n = 22x). The 2C DNA content positively correlated with the ploidy status and stomatal length (r = 0.814, p < 0.001). Based on the 1Cx value, the study also suggests that the majority of the polyploid species have experienced genome downsizing in relation to their diploid progenitors. This study provides the most essential information on chromosome number, ploidy and DNA content to facilitate the uti-lization of a core subset of germplasm in the mulberry breeding program.
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