Comparative analysis of the occupancy of Histone H3 Lysine 4 methylation in the cells treated with TGF beta and Interferon gamma

In this current study, we have compared our H3K4me3 Chip-Sequencing data in PC3 cells in response to 6 h and 24 h TGF beta stimulation with the IFN gamma stimulated/unstimulated HeLa S3 cells Since both TGF beta and IFN gamma play an essential role in tumorigenesis both as a tumor promoter and tumor suppressor and known to antagonize each other's signalling, it would be of utmost importance to find out the regions undergoing histone modification changes in response to TGF beta and IFN gamma and compare them to explore the genes common to both as well as the specific for each ligand. Our study has compared the genes showing H3K4me3 occupancy in response to both TGF beta and IFN gamma. Several genes were found to be shared between the TGF beta and IFN gamma. DAVID Functional enrichment analysis in the TGF beta and IFN gamma dataset revealed association of genes with different biological processes such as miRNA-mediated gene silencing, positive regulation of ERK cascade, hypoxia-induced apoptosis repression, translational regulation and molecular functions such as TGF beta R activity, GPCR activity, TGF beta binding activity. Further analysis of these genes can reveal fascinating insights into epigenetic regulation by growth factor stimulation.

Automated summary

By comparing H3K4me3 Chip-Sequencing data in response to TGF beta and IFN gamma stimulation, we identified genes that are regulated by both ligands. These findings are important for understanding the signaling pathways and specific gene functions related to tumorigenesis.