期刊
CHEMICAL COMMUNICATIONS
卷 51, 期 12, 页码 2208-2224出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4cc09218j
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资金
- LOEWE Research Cluster SynChemBio
- Max-Planck-Society
Cytochrome P450 enzymes (CYPs) have been used for more than six decades as catalysts for the CH-activating oxidative hydroxylation of organic compounds with formation of added-value products. However, it has not been possible to control regio- and stereoselectivity in a general manner, which is necessary for wide (industrial) applications. Especially directed evolution as a Darwinian approach to protein engineering has changed the situation for the better during the last 3-4 years leading to extensive progress, which is summarized and analysed in this Feature Article.
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