Construction of a LAMP-CRISPR assay for the detection of Vibrio parahaemolyticus

Article Chemistry, Analytical

Filtration-based LAMP-CRISPR/Cas12a system for the rapid, sensitive and visualized detection of Escherichia coli O157:H7

So-Young Lee et al.

Summary: In this study, a rapid, sensitive, and visualized method of detecting E. coli O157:H7 in fresh products was developed, which rectified the common false-negative results and had a high detection sensitivity.

TALANTA (2022)

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Article Food Science & Technology

CRISPR/Cas12a-based technology: A powerful tool for biosensing in food safety

Zefeng Mao et al.

Summary: The use of molecular detection in food safety has become urgent due to the detection of novel coronavirus events in the food cold chain. This review introduces a new molecular diagnostic technology based on CRISPR-Cas12a and summarizes its applications in various aspects of food safety. The biosensor analytical technology has great potential in qualitative and quantitative analysis of infectious pathogens.

TRENDS IN FOOD SCIENCE & TECHNOLOGY (2022)

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Review Plant Sciences

Clustered Regularly Interspaced Short Palindromic Repeats-Associated Protein System for Resistance Against Plant Viruses: Applications and Perspectives

Fredy D. A. Silva et al.

Summary: This paper presents recent advances in engineering resistance against plant viruses using the CRISPR/Cas system, as well as the potential of the CRISPR/Cas system for targeting host factors, achieving broad-range resistance, and enhancing immunity. It also outlines the applications, challenges, and perspectives of the CRISPR/Cas system for crop protection.

FRONTIERS IN PLANT SCIENCE (2022)

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Article Biophysics

Argonaute-integrated isothermal amplification for rapid, portable, multiplex detection of SARS-CoV-2 and influenza viruses

Xingyu Ye et al.

Summary: In this study, a multiplex nucleic acid detection system called MULAN was developed, which integrates rapid isothermal amplification with the multiplex inclusiveness of a single Ago for simultaneous detection of multiple viruses. MULAN showed high specificity and sensitivity, and could be used for portable and visible detection.

BIOSENSORS & BIOELECTRONICS (2022)

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Article Microbiology

Multilocus Sequence Typing and Virulence Potential of Vibrio parahaemolyticus Strains Isolated from Aquatic Bird Feces

Chonchanok Muangnapoh et al.

Summary: This study isolated and characterized Vibrio parahaemolyticus from aquatic bird feces and analyzed their genetic diversity. The results showed that aquatic birds are natural reservoirs of V. parahaemolyticus with high genetic diversity and may play a role in the dissemination of potentially pathogenic strains.

MICROBIOLOGY SPECTRUM (2022)

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Article Chemistry, Applied

RPA-Cas12a-FS: A frontline nucleic acid rapid detection system for food safety based on CRISPR-Cas12a combined with recombinase polymerase amplification

Hua Liu et al.

Summary: This study developed a detection technique by combining recombinase polymerase amplification with CRISPR-Cas12a for food safety, which can rapidly, simply, and highly sensitively detect foodborne pathogenic bacteria, genetically modified crops, and meat adulteration without requiring technical expertise or equipment.

FOOD CHEMISTRY (2021)

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Article Chemistry, Analytical

A novel photoelectrochemical aptamer sensor based on rare-earth doped Bi2WO6 and Ag2S for the rapid detection of Vibrio parahaemolyticus

Yunxia Hou et al.

Summary: In this study, a photoelectrochemical (PEC) aptamer sensor was constructed using a layer-by-layer self-assembly method, with rare-earth doped Bi2WO6 and Ag2S as photoactive materials to enhance photocurrent responsiveness under visible light. The optimized experimental conditions showed high detection specificity and stability for Vibrio parahaemolyticus, indicating the potential of this method for rapid detection of other food-borne pathogenic bacteria.

MICROCHEMICAL JOURNAL (2021)

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Article Biophysics

Fluorescence polarization system for rapid COVID-19 diagnosis

Chang Yeol Lee et al.

Summary: CODA is a portable system for fast, on-site diagnosis of COVID-19, with high signal-to-noise ratio that can complete testing within 20 minutes and has a low limit of detection.

BIOSENSORS & BIOELECTRONICS (2021)

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Review Food Science & Technology

Food safety issues in fresh produce supply chain with particular reference to sub-Saharan Africa

O. Charles Aworh

Summary: Developing countries, especially sub-Saharan Africa, face the greatest burden of food-borne illnesses with animal-source foods and fresh produce being the leading causes. Pathogenic bacteria, viruses, and chemical contaminants are the primary agents of food-borne illnesses in SSA. Improving food safety practices, post-harvest handling, market infrastructure, and technology adoption can enhance food safety in SSA.

FOOD CONTROL (2021)

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Article Multidisciplinary Sciences

An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing

Kean Hean Ooi et al.

Summary: Extensive testing is crucial to control the spread of SARS-CoV-2 in the ongoing COVID-19 pandemic. The authors have developed a CRISPR-based diagnostic assay that can detect both wildtype and known variants of the virus efficiently and quickly.

NATURE COMMUNICATIONS (2021)

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Article Microbiology

Longitudinal Study of Total and Pathogenic Vibrio parahaemolyticus (tdh plus and/or trh plus ) in Two Natural Extraction Areas of Mytilus chilensis in Southern Chile

Cristina Bacian et al.

Summary: Vibrio parahaemolyticus is the leading cause of seafood-associated bacterial gastroenteritis worldwide. This study determined the prevalence of V. parahaemolyticus in mussels from two natural growing areas in Chile, finding higher detection rates in the Valdivia River Estuary compared to the Reloncavi Fjord, with significant influence from seasonal water temperature and area factors.

FRONTIERS IN MICROBIOLOGY (2021)

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Article Biophysics

Microfluidic thread-based electrochemical aptasensor for rapid detection of Vibrio parahaemolyticus

Hai Jiang et al.

Summary: The thread-based microfluidic electrochemical aptasensor is designed for rapid and highly sensitive detection of Vibrio parahaemolyticus in seafood. It has higher detection sensitivity and shorter assay time compared to traditional methods.

BIOSENSORS & BIOELECTRONICS (2021)

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Review Microbiology

Environmental parameters associated with incidence and transmission of pathogenic Vibrio spp.

Kyle D. Brumfield et al.

Summary: Vibrio spp. are bacteria that thrive in warm water and moderate salinity, some of which are pathogenic to humans, such as Vibrio cholerae. The global incidence of Vibrio infections is on the rise, closely linked to factors like climate change.

ENVIRONMENTAL MICROBIOLOGY (2021)

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Article Chemistry, Analytical

LAMP-CRISPR-Cas12-based diagnostic platform for detection of Mycobacterium tuberculosis complex using real-time fluorescence or lateral flow test

Yi Wang et al.

Summary: The LACD system utilizes engineered core primers with PAM sites to enable CRISPR-Cas12a mediated detection in the LAMP stage.

MICROCHIMICA ACTA (2021)

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Review Microbiology

Antimicrobial Properties of Food Nanopackaging: A New Focus on Foodborne Pathogens

Amir Ali Anvar et al.

Summary: Food products contaminated by foodborne pathogens can cause diseases, and efforts are being made to develop novel agents to combat these microorganisms, which may have implications for the COVID-19 pandemic. Nanotechnology has introduced a new food packaging technology utilizing nanomaterials with unique antimicrobial properties to extend food shelf life.

FRONTIERS IN MICROBIOLOGY (2021)

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Article Food Science & Technology

Burden of foodborne diseases: think global, act local

Sara M. Pires et al.

Summary: National burden of foodborne disease studies are crucial for establishing food safety as a public health priority, ranking diseases, and informing interventions. Despite progress in implementing these studies, the current landscape of disease burden remains disjointed, making it challenging for researchers to translate findings into policy recommendations.

CURRENT OPINION IN FOOD SCIENCE (2021)

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Article Chemistry, Multidisciplinary

Rapid detection of Vibrio parahaemolyticus using magnetic nanobead-based immunoseparation and quantum dot-based immunofluorescence

Yue Zhai et al.

Summary: This study presents a rapid and sensitive detection method for Vibrio parahaemolyticus by integrating magnetic nanobeads and quantum dots technology. The detection combines immunoseparation and immunofluorescence to achieve efficient detection of Vibrio parahaemolyticus, showing satisfactory results in real food samples.

RSC ADVANCES (2021)

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Review Chemistry, Analytical