Multi-immunoaffinity column for the simultaneous analysis of bisphenol A and its analogues in Chinese foods by liquid chromatography tandem mass spectrometry

Bisphenol A (BPA) and its four analogues have been receiving considerable attention owing to their potential endocrine disrupting effects. The European Food Safety Authority has proposed 0.04 ng/kg center dot body weight/day of the temporary tolerable daily intake for BPA. Therefore, a more sensitive analytical method was urgently needed for the necessity of the risk reassessment of bisphenols (BPs). The matrix effect of Chinese foods is a challenge for the analysis of ultra-trace analytes due to the presence of various spices. A multi-immunoaffinity column (mIAC) was prepared for the purification of BPA, BPB, BPF, BPS, and BPAF in Chinese foods following ultra -high-performance liquid chromatography tandem mass spectrometry detection (UHLPC-MS/MS). The recoveries of each of BPs were ranged from 84.6% to 116.7%, and the intra-day precision and inter-day precision were ranged from 1.6% to 12.4%, and from 4.1% to 14.0%, respectively. This is the first report on the mIACs for simultaneous clean-up and analysis of BPs in complex Chinese foods.

Automated summary

The European Food Safety Authority (EFSA) has proposed a temporary tolerable daily intake of 0.04 ng/kg body weight/day for Bisphenol A (BPA) and its four analogues due to their potential endocrine disrupting effects. A more sensitive analytical method was urgently needed for the risk reassessment of bisphenols (BPs). The matrix effect of Chinese foods, which contain various spices, is a challenge for the analysis of ultra-trace analytes. In this study, a multi-immunoaffinity column (mIAC) combined with ultra-high-performance liquid chromatography tandem mass spectrometry detection (UHLPC-MS/MS) was used for the purification and analysis of BPA, BPB, BPF, BPS, and BPAF in Chinese foods.