Development of a novel functional yogurt rich in lycopene by Bacillus subtilis

Functional lycopene-rich yogurt displays attractive nutritious and health-promoting benefits among existing functional dairy products, owing to supplement with lycopene which could enhance immunity, prevent cancer, and cardiovascular diseases. Due to poor stability and fat-solubility of lycopene, its incorporation into yogurt is challengeable. In this study, carotenoid genes for lycopene synthesis were co-introduced into probiotic Bacillus subtilis for efficient lycopene production. Further engineered B. subtilis was applied as adjunct starter culture for achieving lycopene-rich yogurt. Developed yogurt exhibited desirable physiochemical characteristics compared with plain yogurt. Moreover, lycopene-rich yogurt was endowed with significantly high antioxidant capacity. More importantly, this functionalized yogurt had attractive sensorial attributes for quality-assured food to facilitate consumer acceptance. As the first report of fortifying yogurt of lycopene using B. subtilis with improved functional properties, this study offers a new and facile clue to enrich bioactive lycopene and probiotic B. subtilis in yogurt for healthy and nutritional food development.

Automated summary

In this study, carotenoid genes for lycopene synthesis were introduced into probiotic Bacillus subtilis to produce lycopene-rich yogurt. The developed yogurt not only had desirable physiochemical characteristics compared with plain yogurt, but also exhibited significantly high antioxidant capacity. This study offers a new and facile clue to enrich bioactive lycopene and probiotic Bacillus subtilis in yogurt for healthy and nutritional food development.