Multi-residue method to determine selected personal care products from five classes in fish based on miniaturized matrix solid-phase dispersion and solid-phase microextraction coupled to gas chromatography-mass spectrometry

A method featuring matrix solid-phase dispersion combined with solid-phase microextraction coupled to gas chromatography-mass spectrometry was developed to determine parabens, musks, antimicrobials, UV filters, and an insect repellent in fish. Optimization and validation of the method was carried out on tilapia and salmon samples. Acceptable linearity (R2 > 0.97), precision (relative standard deviations < 13 %) and accuracy (recovery > 80 %) at two concentration levels for all analytes were obtained using both matrices. The limits of detection ranged from 0.01 to 1.01 mu g g-1 (wet weight) for all analytes except for methyl paraben. The SPME Arrow format was applied to increase the sensitivity of the method, and yielded detection limits more than ten times lower than those achieved with traditional SPME. The miniaturized method can be applied to various fish species, regardless of their lipid content, and represents a useful tool for quality control and food safety purposes.

Automated summary

A method combining matrix solid-phase dispersion and solid-phase microextraction coupled to gas chromatography-mass spectrometry was developed for analyzing parabens, musks, antimicrobials, UV filters, and an insect repellent in fish. The method was optimized and validated using tilapia and salmon samples. The miniaturized method showed good linearity, precision, and accuracy for all analytes, and the detection limits were significantly improved using the SPME Arrow format.