Immunomodulation of RAW264.7 cells by CP80-1, a polysaccharide of Cordyceps cicadae, via Dectin-1/Syk/NF-κB signaling pathway

Cordyceps cicadae is a rare edible and medicinal entomogenous fungus. Here, the structural characteristics and immunomodulatory activity of C. cicadae polysaccharide CP80-1 were preliminarily elucidated. It showed that CP80-1 of 250.431 kDa was mainly composed of glucose, xylose and rhamnose. Physiologically, CP80-1 can regulate the immune of RAW264.7 cells by promoting the proliferation, phagocytosis, and secretion of nitric oxide (NO), tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta, and interleukin (IL)-6. However, these effects were significantly attenuated when Dectin-1 receptor expression was inhibited by laminarin. Moreover, laminarin down-regulated the mRNA levels of relevant genes, inhibited CP80-1-driven expression of proteins related to spleen tyrosine kinase (Syk) and nuclear factor kappa-B (NF-.B) signaling pathway. Additionally, CP80-1 induced the nuclear translocation of NF-.B subunit p65 in RAW264.7 cells. These results suggest that CP80-1 may activate RAW264.7 cells by regulating the Dectin-1/ Syk/NF-.B signaling pathway, which provides theoretical basis for the development of C. cicadae in immunomodulation.

Automated summary

Research has shown that Cordyceps cicadae polysaccharide CP80-1 can regulate the immune system by modulating the functions of immune cells. It promotes cell proliferation, phagocytosis, and the production of various immune molecules. This regulatory effect is likely mediated through specific signaling pathways.