Molecular cytogenetic analysis of the olive flounder embryonic cell line FGBC8 and its applicability to biotechnology

We explored the biotechnological applicability of a previously established olive flounder (Paralichthys olivaceus) embryonic cell line (FGBC8). FGBC8 was transfected with pEGFP-c1 and pluripotency-related genes, then infected with viral hemorrhagic septicemia virus (VHSV), and the expression of immune-related genes was observed through quantitative real-time polymerase chain reaction. Transfected cells showed strong green fluorescence 48 h after transfection, and pluripotency-related genes were successfully transfected. In addition, FGBC8 cells were highly susceptible to VHSV and the expression of immune-related genes was induced during infection. Our results demonstrate that FGBC8 cells are valuable research tools for assessing host-pathogen interactions and biotechnological applications.

Automated summary

We explored the potential biotechnological applications of an established olive flounder embryonic cell line (FGBC8). The cells were transfected with pEGFP-c1 and pluripotency-related genes, and then infected with viral hemorrhagic septicemia virus (VHSV). The expression of immune-related genes was observed, indicating the value of FGBC8 cells in studying host-pathogen interactions and biotechnological applications.