期刊
METABOLISM-CLINICAL AND EXPERIMENTAL
卷 65, 期 3, 页码 54-63出版社
W B SAUNDERS CO-ELSEVIER INC
DOI: 10.1016/j.metabol.2015.10.021
关键词
beta-cell; Bile acids; Insulin secretion; TUDCA
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo [FAPESP 2013/01318-4]
- Conselho Nacional para o Desenvolvimento Cientifico e Tecnologico [CNPq 200030/2014-0]
- Instituto Nacional de Obesidade e Diabetes (CNPq/FAPESP)
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
- Spanish Ministerio de Ciencia e Innovacion [BFU2013-42789-P, BFU2011-28358]
Objective. While bile acids are important for the digestion process, they also act as signaling molecules in many tissues, including the endocrine pancreas, which expresses specific bile acid receptors that regulate several cell functions. In this study, we investigated the effects of the conjugated bile acid TUDCA on glucose-stimulated insulin secretion (GSIS) from pancreatic beta-cells. Methods. Pancreatic islets were isolated from 90-day-old male mice. Insulin secretion was, measured by radioimmunoassay, protein phosphorylation by western blot, Ca2+ signals by fluorescence microscopy and ATP-dependent K+ (K-ATP) channels by electrophysiology. Results. TUDCA dose-dependently increased GSIS in fresh islets at stimulatory glucose concentrations but remained without effect at low glucose levels. This effect was not associated with changes in glucose metabolism, Ca2+ signals or K-ATP channel activity; however, it was lost in the presence of a cAMP competitor or a PICA inhibitor. Additionally, PKA and CREB phosphorylation were observed after 1-hour incubation with TUDCA. The potentiation of GSIS was blunted by the G alpha stimulatory, G protein subunit-specific inhibitor NF449 and mimicked by the specific TGRS agonist INT-777, pointing to the involvement of the bile acid G protein-coupled receptor TGRS. Conclusion. Our data indicate that TUDCA potentiates GSIS through the cAMP/PKA pathway. (C) 2015 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据