期刊
METABOLIC ENGINEERING
卷 38, 期 -, 页码 56-64出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2016.06.005
关键词
RuBisCO; CcmM; SBPase; FBA; TK; Synechocystis PCC 6803
资金
- Nordic Energy Research (project AquaFeed) [NER-24]
- Knut and Alice Wallenberg Foundation (project MoSE) [2011.0067]
- Swedish Energy [11674-5]
- Chinese Service Center for Scholarly Exchange [201304910361]
Synechocystis PCC 6803 is a model unicellular cyanobacterium used in e.g. photosynthesis and CO2 assimilation research. In the present study we examined the effects of overexpressing Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), sedoheptulose 1,7-biphosphatase (SBPase), fructose-bisphosphate aldolase (FBA) and transketolase (TK), confirmed carbon flux control enzymes of the Calvin-Bassham-Benson (CBB) cycle in higher plants, in Synechocystis PCC 6803. Overexpressing RuBisCO, SBPase and FBA resulted in increased in vivo oxygen evolution (maximal 115%), growth rate and biomass accumulation (maximal 52%) under 100 ttmol photons m(-2) s(-1) light condition. Cells overexpressing TK showed a chlorotic phenotype but increased biomass by approximately 42% under 100 ttmol photons m(-2) s(-1) light condition. Under 15 ttmol photons m(-2) s(-1) light condition, cells over expressing TK showed enhanced in vivo oxygen evolution. This study demonstrates increased growth and biomass accumulation when overexpressing selected enzymes of the CBB cycle. RuBisCO, SBPase, FBA and TK are identified as four potential targets to improve growth and subsequently also yield of valuable products from Synechocystis PCC 6803. (C) 2016 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据