The role of cytochrome P450 3A2 and 4V2 in response to high-temperature stress in Tetranychus truncatus (Acari: Tetranychidae)

Temperature is an important factor influencing the physiological activities of agricultural pests. Therefore, understanding pest physiological activities and the molecular response to high-temperature stress is of paramount importance for pest management. Tetranychus truncatus Ehara (Acari: Tetranychidae) is a harmful organism, that may cause serious harm to crops such as corn and cotton in high-temperature environments. Cytochrome P450 (CYP450) is induced by high-temperature stress, and it plays an important role in the resistance of spider mites to high temperatures. Because of their role in high-temperature tolerance, the cytochrome P450 genes of the spider mite are attracting more and more attention. In this study, we identified and analyzed CYP450 genes in T. truncatus to investigate their potential roles in growth and development and the resistance to high-temperature stress. Based on phylogenetic and structural analyses, we identified 17 CYP450 genes in T. truncatus. RNA-seq and real-time quantitative PCR (RT-qPCR) revealed differential expression patterns of these genes at different developmental stages and levels of high-temperature stress resistance. The RNA interference results of selected CYP450 showed that when TtCYP3A2 and TtCYP4V2 were silenced by feeding on dsRNA, respectively, the high-temperature resistance of T. truncatus was decreased, which indicated that the expression of these two CYP450 genes in this species may be related to high-temperature tolerance. Our results provide potential evidence for the response of spider mites to high-temperature stress and help to improve the understanding of T. truncatus's ability to resist high-temperature stress.

Automated summary

Temperature is a crucial factor affecting the physiological activities of agricultural pests. This study investigates the molecular response of Tetranychus truncatus to high-temperature stress and the potential roles of cytochrome P450 (CYP450) genes in growth, development, and resistance to high temperatures. The results reveal differential expression patterns of CYP450 genes at different developmental stages and levels of high-temperature stress, and demonstrate that the expression of certain CYP450 genes may be related to high-temperature tolerance in T. truncatus.