期刊
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
卷 191, 期 -, 页码 303-314出版社
ELSEVIER
DOI: 10.1016/j.ejpb.2023.09.007
关键词
Thermal protection; Skin penetration prediction; Raman imaging; Betamethasone-17-valerate skin distribution; Multi -lamellar liposomes
Our previous work has found that the size, elasticity, and charge of multi-lamellar liposomes (MLLs) cannot be considered independently when predicting their fate in the skin. In this study, we developed MLLs formulations containing the corticosteroid betamethasone 17-valerate (B17) to deliver the drug to specific layers of the skin. The MLLs showed high encapsulation efficiency and protected the corticosteroid from thermal degradation. We established an equation to determine the penetration depth of MLLs in artificial skin, and confirmed its accuracy through experiments on human skin samples.
Our previous work showed that the size, elasticity and charge of multi-lamellar liposomes (MLLs) could not be considered separately to predict the fate of MLLs in the skin [1]. Based on this study, we developed several MLLs formulations containing a corticosteroid, betamethasone 17-valerate (B17) to transport the drug into the stratum corneum, living epidermis, dermis or through the skin. MLLs encapsulation efficiency was found to exceed 74 +/- 3 % in all cases. In addition, we showed that MLLs protected the corticosteroid from thermal degradation. Comparing the penetration depth of all MLLs within artificial skin measured by Raman imaging, we established an equation for its determination, given the MLLs elasticity and size. This equation was verified experimentally on human explants: quantification of B17 in each skin layer, as well as its transdermal passage by ultra-high performance liquid chromatography, confirmed that B17 was predominantly and significantly transported in the desired layer. Eventually, we showed the benefits in using B17-loaded MLLs instead of a B17-containing pharmaceutical cream in terms of B17 penetration and thermal degradation.
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