4.7 Article

Discovery of new imidazotetrazinones with potential to overcome tumor resistance

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ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.ejmech.2023.115507

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Nitrogen heterocycles; Imidazotetrazinones; Tumor growth inhibition of glioblastoma; DMPK studies

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This study describes the design, synthesis, and characterization of novel analogues of temozolomide, a clinically used antitumor agent. The new compounds exhibit enhanced growth-inhibitory activity against various human tumor cell lines, including those that are resistant to temozolomide. Through rational design, the incorporation of a propargyl alkylating moiety and a thiazole ring as a replacement for a carboxamide has been achieved, resulting in potent and stable compounds. The findings suggest that further preclinical evaluation of the thiazole analogue is warranted.
We describe the design, organic synthesis, and characterization, including X-ray crystallography, of a series of novel analogues of the clinically used antitumor agent temozolomide, together with their in vitro biological evaluation. The work has resulted in the discovery of a new series of anticancer imidazotetrazines that offer the potential to overcome the resistance mounted by tumors against temozolomide. The rationally designed com-pounds that incorporate a propargyl alkylating moiety and a thiazole ring as isosteric replacement for a car-boxamide, are readily synthesized (gram-scale), exhibit defined solid-state structures, and enhanced growth -inhibitory activity against human tumor cell lines, including MGMT-expressing and MMR-deficient lines, mo-lecular features that confer tumor resistance. The cell proliferation data were confirmed by clonogenic cell survival assays, and DNA flow cytometry analysis was undertaken to determine the effects of new analogues on cell cycle progression. Detailed 1H NMR spectroscopic studies showed that the new agents are stable in solution, and confirmed their mechanism of action. The propargyl and thiazole substituents significantly improve potency and physicochemical, drug metabolism and permeability properties, suggesting that the thiazole 13 should be prioritized for further preclinical evaluation.

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