Short-term in vitro exposure of Pinctada imbricata's haemocytes to quaternium-15: Exploring physiological and cellular responses

Since the 2000 s, the pearl oyster Pinctada imbricata (Ro & BULL;ding, 1798) has become established along the transitional waterways of the Capo Peloro Lagoon natural reserve, where it is now abundant due to its adaptability to different hydrological, climatic, environmental, and pollution conditions. This study aims to evaluate haemocyte immune-mediated responses in vitro to quaternium-15, a common pollutant in aquatic ecosystems. Cell viability and phagocytosis activity decreased when exposed to 0.1 or 1 mg L- 1 of quaternium-15. Moreover, decreasing phagocytosis was confirmed by gene expression modulation of actin, involved in cytoskeleton rearrangement. Effects on oxidative stress-related genes were also assessed (Cat, MnSod, Zn/CuSod, GPx). The qPCR data revealed alterations in antioxidant responses through gene dose- and time-dependent modulation. This study presents insights into the physiological responses and cellular mechanisms of P. imbricata haemocytes to environmental stressors, indicating that this species is useful as a novel bioindicator for future toxicological studies.

Automated summary

Since the 2000s, the pearl oyster Pinctada imbricata has thrived in the transitional waterways of the Capo Peloro Lagoon natural reserve. This study investigates the immune-mediated responses of P. imbricata haemocytes to the aquatic pollutant Quaternium-15. Exposure to Quaternium-15 resulted in decreased cell viability, phagocytosis activity, and alterations in antioxidant responses. The findings suggest that P. imbricata could serve as a bioindicator for future toxicological studies.