期刊
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
卷 104, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.etap.2023.104298
关键词
Nanoplastics; Intestine; Inflammation; TLR4; Microglia; p38
Nanoplastics have the potential to activate inflammatory pathways in human cells, leading to upregulation of proteins and increased production of inflammatory factors. The response varies depending on the cell type.
The crescent presence of nanoplastics in the environment raises concerns regarding their potential impact on health. This study exposed human colon adenocarcinoma cells (HT29) and microglia cells (N9) to nanoplastics (25 nm, 50 nm, and 100 nm Polystyrene) to investigate their inflammatory responses, which are vital for body's defence. Although cytotoxicity remained generally low, HT29 cells exhibited a notable upregulation of p50 and p38 expression, concomitant with elevated TLR4 expression, in contrast with N9 cells that showed a less pro-nounced upregulation of these proteins. Additionally, nanoplastic exposure increased IL-1ss levels, partially attenuated by pre-exposure to TLR4 or p38 inhibitors. Intriguingly, N9 cells exposed to nanoplastics exhibited substantial increases in iNOS mRNA. This effect was entirely prevented by pre-exposure to TLR4 or p38 in-hibitors, while TNF-alpha mRNA levels remained relatively stable. These findings underscore the potential of nanoplastics to activate inflammatory pathways, with response kinetics varying depending on the cell type.
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