Combination of sulfide-driven partial denitrification with anammox enhanced by zeolite powder for autotrophic nitrogen and sulfide removal from wastewater

Sulfide-driven partial denitrification and anaerobic ammonia oxidizing (anammox) (SPDA) is a high-efficiency technology to achieve simultaneous nitrogen and sulfide removal. Nitrite accumulation from sulfide-driven partial denitrification is the key to achieve SPDA. Zeolite powder was added to strengthen the competition of anammox bacteria against nitrite. The nitrogen removal rate (NRR) and partial denitrification efficiency in reactor was 5.18 kg-N m � 3d-1 and 92.3% during 180 days of operation, higher than those without zeolite powder, indicating an improving contribution of zeolite powder. Metabolomics analysis revealed zeolite powder addition enhanced the metabolisms of amino acids, nicotinate and porphyrin through increasing glutamate content, and improved EPS secretion, heme c content and particle size. Besides, high ammonia enriched by zeolite powder was conducive to improve anammox activity and NRR. This study provides the metabolic insights into the mechanism of zeolite powder enhancing SPDA, which is meaningful towards overcoming the limitations in practical application of SDPA.

Automated summary

This study finds that the addition of zeolite powder enhances the competition between anammox bacteria and nitrite in the SPDA process, resulting in improved nitrogen removal. Zeolite powder enrichment promotes various metabolic pathways and improves particle characteristics, leading to enhanced nitrogen removal efficiency. The high ammonia concentration enriched by zeolite powder also positively affects anammox activity.