Antimicrobial, antioxidant, anticancer, and antithrombotic, competency of saponins from the root of Decalepis hamiltonii

The goal of this study was to extract saponins from the tuberous root of Decalepis hamiltonii and assess their potential clinical applications, which included antioxidant, antibacterial, antithrombotic, and anticancer prop-erties. Surprisingly, the results of this study revealed that the extracted saponins have excellent antioxidant activities, as demonstrated by 2,2-diphenylpicrylhydrazyl (DPPH), 2,2 '-azino-bis(3-ethylbenzothiazoline-6-sul-fonic acid) (ABTS), Hydrogen peroxide (H2O2), and Nitric oxide (NO) scavenging assays. Nonetheless, at a concentration of 100 g/mL, crude saponin had excellent antibacterial activity, particularly against gramme positive bacteria (Staphylococcus aureus, Bacillus subtilis, Staphylococcus epidermidis, and Micrococcus luteus), fol-lowed by gramme negative bacteria (Escherichia coli, Salmonella typhi, Proteus mirabilis, and Klebsiella pneumonia). Despite this, the crude saponin had no effect on Aspergillus niger and Candida albicans. The crude saponin also possesses outstanding in vitro antithrombotic activity on blood clot. Interestingly, the crude saponins have an outstanding anticancer activity of 89.26%, with an IC50 value of 58.41 mu g/mL. Overall, the findings conclude that crude saponin derived from D. hamiltonii tuberous root could be used in pharmaceutical formulations.

Automated summary

The study aimed to extract saponins from Decalepis hamiltonii and explore their potential clinical applications, including antioxidant, antibacterial, antithrombotic, and anticancer properties. The findings revealed that the extracted saponins exhibited excellent antioxidant activities and significant antibacterial effects against gram-positive and gram-negative bacteria. Moreover, the crude saponins demonstrated remarkable antithrombotic activity and strong anticancer activity. These results suggest that crude saponin derived from D. hamiltonii could be used in pharmaceutical formulations.