Potential of direct granulation and organic loading rate tolerance of aerobic granular sludge in ultra-hypersaline environment

Salt-tolerant aerobic granular sludge (SAGS) technology has shown potentials in the treatment of ultra-hypersaline high-strength organic wastewater. However, the long granulation period and salt-tolerance accli-mation period are still bottlenecks that hinder SAGS applications. In this study, one-step development strategy was used to try to directly cultivate SAGS under 9% salinity, and the fastest cultivation process was obtained under such high salinity compared to the previous papers with the inoculum of municipal activated sludge without bioaugmentation. Briefly, the inoculated municipal activated sludge was almost discharged on Day 1-10, then fungal pellets appeared and it gradually transitioned to mature SAGS (particle size of-4156 mu m and SVI30 of 57.8 mL/g) from Day 11 to Day 47 without fragmentation. Metagenomic revealed that fungus Fusarium played key roles in the transition process probably because it functioned as structural backbone. RRNPP and AHL-mediated systems might be the main QS regulation systems of bacteria. TOC and NH4+-N removal effi-ciencies maintained at-93.9% (after Day 11) and-68.5% (after Day 33), respectively. Subsequently, the influent organic loading rate (OLR) was stepwise increased from 1.8 to 11.7 kg COD/m3 center dot d. It was found that SAGS could maintain intact structure and low SVI30 (< 55 mL/g) under 9% salinity and the OLR of 1.8-9.9 kg COD/m3 center dot d with adjustment of air velocity. TOC and NH4+-N (TN) removal efficiencies could maintain at-95.4% (below OLR of 8.1 kg COD/m3 center dot d) and-84.1% (below nitrogen loading rate of 0.40 kg N/m3 center dot d) in ultra-hypersaline environment. Halomonas dominated the SAGS under 9% salinity and varied OLR. This study confirmed the feasibility of direct aerobic granulation in ultra-hypersaline environment and verified the upper OLR boundary of SAGS in ultra-hypersaline high-strength organic wastewater treatment.

Automated summary

Salt-tolerant aerobic granular sludge (SAGS) technology has the potential to treat ultra-hypersaline high-strength organic wastewater. This study used a one-step development strategy to directly cultivate SAGS under 9% salinity, achieving the fastest cultivation process compared to previous studies. The transition process was mainly driven by the fungus Fusarium, with RRNPP and AHL-mediated systems as the main regulation systems of bacteria. SAGS exhibited high removal efficiencies of TOC and NH4+-N under ultra-hypersaline conditions, and Halomonas dominated the SAGS.