期刊
ENVIRONMENTAL RESEARCH
卷 237, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.envres.2023.116928
关键词
Bacteria survival; Microcosms; Competition; Native microbiota
This study compared the behavior of a clinical and an environmental K. pneumoniae strain harboring the blaKPC-3 gene in water. The abundance of the genes phoE and blaKPC-3 was monitored in urban runoff water and sterile ultra-pure water microcosms to assess survival, blaKPC-3 persistence, and the effect of the water microbiota. The results showed that K. pneumoniae and the blaKPC-3 gene decreased in urban runoff water, indicating competition with native microbiota, but the bacteria could still survive for at least one week.
Carbapenem-resistant Klebsiella pneumoniae represents a healthcare threat, already disseminated in the environment. This study aimed to compare the behaviour of a clinical and an environmental K. pneumoniae strain (multilocus sequence type ST147) harbouring the gene blaKPC-3 in water. The abundance of the genes phoE (specific for K. pneumoniae) and blaKPC-3 was monitored by quantitative PCR in urban runoff water and sterile ultra-pure water microcosms, aiming to assess survival, blaKPC-3 persistence, and the effect of the native water microbiota. In sterile ultra-pure water, the abundance of cultivable K. pneumoniae and blaKPC-3 gene did not change over the incubation period (8 days). In contrast, in urban runoff, the K. pneumoniae and the genes phoE and blaKPC genes decreased by up to 3 log-units. These results suggest that K. pneumoniae were outcompeted by the native microbiota of the urban runoff water and that the decay of blaKPC-3 gene was due to host death, rather than to gene loss. The study highlights that although native microbiota is essential to hamper the persistence of non-native bacteria, carbapenemase producing K. pneumoniae can survive in urban runoff water for at least one week.
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