Road salt pollution alters sex ratios in emerging mosquito populations*

De-icing road salt is a persistent emerging pollutant in temperate freshwater systems, where winter salting is necessary for road and pedestrian safety. Experts argue that road salts may increase salt-tolerant mosquito populations and, potentially, disease transmission in urban areas. Only adult females consume bloodmeals and may carry zoonotic diseases. While there are some species with naturally occurring male-biased sex ratios, it is unclear whether road salt differentially affects male and female mosquitoes to alter sex ratios. We hypothesized that road salts would masculinize emergence sex ratios and decrease female success because females may face higher exposure to stressors during their lengthy juvenile development compared to males. We measured mosquito emergence sex ratios of control (0 g/L added salt) and salt (4.5 g/L added salt) mesocosms in southern Ontario, Canada across the West Nile Virus season (May to October). We found female-biased sex ratios (i.e., <50% male frequency) in both 0 and 4.5 g/L. While mosquito abundance was significantly higher in 4.5 g/L compared to 0 g/L, road salt significantly increased the proportion of emerging males from 32.8% to 40.8% (Negative Binomial Model; Estimate & PLUSMN; SE = 0.283 & PLUSMN; 0.108; P = 0.009); mosquitoes shift their sex ratios from female-biased towards parity (50:50) in response to salt. Our study illustrates the need to evaluate sex-specific abundance in pollution-related mosquito population studies. By showing a shift toward more male mosquitoes emerging in high salinity compared to control treatments, our results suggest that road salts may have the potential to decrease female mosquito success and indirectly reduce disease transmission in cities.

Automated summary

De-icing road salt, necessary for safety in temperate freshwater systems, is found to be an emerging pollutant that may increase salt-tolerant mosquito populations and disease transmission in urban areas. This study found that road salt masculinizes the emergence sex ratios of mosquitoes and increases the proportion of males, potentially decreasing female mosquito success and reducing disease transmission in cities.