☆ 4.7 Article

A bispecific nanobody with high sensitivity/efficiency for simultaneous determination of carbaryl and its metabolite 1-naphthol in the soil and rice samples

ENVIRONMENTAL POLLUTION (2023)

相关参考文献

注意：仅列出部分参考文献，下载原文获取全部文献信息。

Article Biochemistry & Molecular Biology

Design of nanobody-based bispecific constructs by in silico affinity maturation and umbrella sampling simulations

Zixuan Bai et al.

Summary: Random mutagenesis is a natural process for protein evolution and can be utilized to create diversity and identify improved variants. Rational mutagenesis, supported by computational power, offers a faster and more predictable strategy to achieve the same goal.

COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL (2023)

添加到收藏夹

Article Engineering, Environmental

Economical amidoxime-functionalized non-porous ?-cyclodextrin polymer for selective detection and extraction of uranium

Yichen Sun et al.

Summary: A dual-functional adsorbent, amidoxime-functionalized non-porous beta-cyclodextrin polymer (AONPCDP), is developed for simultaneous detection and adsorption of U(VI). The adsorbent exhibits selective and efficient quenching of U(VI), resulting in an ultra-low detection limit of 10.3 nM in deionized water. It also shows high sensitivity in detecting U(VI) ions in natural water systems with detection limits of 63.5 nM, 22.2 nM, and 17.6 nM for lake water, seawater, and tap water, respectively. The adsorbent can be easily regenerated and maintains a high reusability of 95.8% after multiple adsorption-desorption cycles.

CHEMICAL ENGINEERING JOURNAL (2023)

添加到收藏夹

Article Chemistry, Analytical

Bispecific Single-Domain Antibodies as Highly Standardized Synthetic Calibrators for Immunodiagnosis

Paula Segovia-de Los Santos et al.

Summary: This study introduces a novel approach using bispecific nanobody technology to generate surrogate recombinant calibrators, overcoming limitations of traditional reference standards in diagnosis of allergies and autoimmune diseases. This technology inherits stability and low-cost production properties, allowing for indefinite reproducibility in a highly standardized manner from its sequence.

ANALYTICAL CHEMISTRY (2022)

添加到收藏夹

Article Nanoscience & Nanotechnology

Preparation of a Nanobody Specific to Dectin 1 and Its Anti-inflammatory Effects on Fungal Keratitis

Xing Liu et al.

Summary: In this study, a nanobody specific to dectin 1 was successfully prepared and showed high-affinity binding to recombinant dectin 1. The nanobody also exhibited specific binding to dectin 1 expressed on the surfaces of cells and recognized dectin 1 in mouse corneas. Importantly, it demonstrated anti-inflammatory effects on Aspergillus fumigatus keratitis.

INTERNATIONAL JOURNAL OF NANOMEDICINE (2022)

添加到收藏夹

Article Biochemistry & Molecular Biology

Development of high-affinity nanobodies specific for NaV1.4 and NaV1.5 voltage-gated sodium channel isoforms

Lakshmi Srinivasan et al.

Summary: In this study, high-affinity anti-Na-V nanobodies (Nbs) Nb17 and Nb82 were generated using phage display technology. These Nbs specifically recognize the Na(V)1.4 and Na(V)1.5 channel isoforms. The results show that Nb17 and Nb82 bind to the CTNa(V)1.4 and CTNa(V)1.5 with high affinity and can detect Na(V)1.4 and Na(V)1.5 channels in mammalian cells and tissues.

JOURNAL OF BIOLOGICAL CHEMISTRY (2022)

添加到收藏夹

Article Medicine, Research & Experimental

CDR loop interactions can determine heavy and light chain pairing preferences in bispecific antibodies

Monica L. Fernandez-Quintero et al.

Summary: Designing different antibody formats, such as bispecific antibodies, is critical for the advancement of the biotherapeutic market. This study investigates the influence of point mutations in the antigen-binding site on heavy and light chain pairing preferences using molecular dynamics simulations. The findings provide mechanistic insights into antibody interfaces and can help advance the design of bispecific antibodies.

MABS (2022)

添加到收藏夹

Article Agriculture, Multidisciplinary

Production and Characterization of Biotinylated Anti-fenitrothionNanobodies and Development of Sensitive Fluoroimmunoassay

Zi-Jian Chen et al.

Summary: A simple and sensitive fluoroimmunoassay based on a heavy-chain antibody was developed for rapid detection of fenitrothion. The assay showed improved sensitivity compared to the conventional enzyme-linked immunosorbent assay and is an ideal tool for rapid screening of fenitrothion in bulk food samples.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2022)

添加到收藏夹

Article Agriculture, Multidisciplinary

Exploration of Specific Nanobodies As Immunological Reagents to Detect Milk Allergen of β-Lactoglobulin without Interference of Hydrolytic Peptides

Yaozhong Hu et al.

Summary: This study successfully developed nanobodies for the immunological detection of intact milk proteins, avoiding interference from hydrolytic peptides.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2022)

添加到收藏夹

Article Biochemical Research Methods

SnoopLigase Enables Highly Efficient Generation of C-C-Linked Bispecific Nanobodies Targeting TNF-α and IL-17A

Jiewen Chen et al.

Summary: This study successfully demonstrated the efficient generation of C-C linked bispecific nanobodies using SnoopLigase, showing potential applications in inhibiting rheumatoid arthritis cell proliferation and migration.

BIOCONJUGATE CHEMISTRY (2022)

添加到收藏夹

Article Engineering, Environmental

Multiplex Lateral Flow Assay and the Sample Preparation Method for the Simultaneous Detection of Three Marine Toxins

Clare Mills et al.

Summary: A multiplex lateral flow immunoassay has been developed to detect marine biotoxins, with specific antibodies for each toxin group. The assay is capable of accurately detecting okadaic acid, saxitoxin, and domoic acid within EU regulatory limits. It offers a simple and portable solution for toxin screening in shellfish.

ENVIRONMENTAL SCIENCE & TECHNOLOGY (2022)

添加到收藏夹

Article Agriculture, Multidisciplinary

Detection of Acrylamide in Foodstuffs by Nanobody-Based Immunoassays

Yifan Liang et al.

Summary: A specific nanobody Nb-7E against acrylamide was isolated and confirmed to be able to detect acrylamide. An ic-ELISA and an ECIA were developed for acrylamide determination, with ECIA showing lower limit of detection and wider linear detection range.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2022)

添加到收藏夹

Article Multidisciplinary Sciences

Development of a bispecific antibody targeting PD-L1 and TIGIT with optimal cytotoxicity

Zhenwei Zhong et al.

Summary: The combination therapy of anti-PD-L1 and anti-TIGIT has shown synergistic efficacy in cancer immunotherapy. Constructing bispecific antibodies targeting PD-L1 and TIGIT can provide new mechanisms of action.

SCIENTIFIC REPORTS (2022)

添加到收藏夹

Review Chemistry, Multidisciplinary

Nanobodies: from structure to applications in non-injectable and bispecific biotherapeutic development

Bingxuan Li et al.

Summary: The increasing demand for convenient, miniaturized and multifunctional antibodies has led to the rapid development of nanobodies as a new tool in biological and medical studies. Nanobodies have advantages such as a stable structure, easy production, high affinity towards antigens, and low immunogenicity. They have been increasingly applied in protein structure analysis, microscopic imaging, medical diagnosis, and drug development. The approval of the first nanobody drug and the outbreak of COVID-19 further accelerated the development of nanobody drugs in non-injectable and bispecific applications.

NANOSCALE (2022)

添加到收藏夹

Article Environmental Sciences

Heterologous expression and exploration of the enzymatic properties of the carbaryl hydrolase CarH from a newly isolated carbaryl-degrading strain

Zhijian Ke et al.

Summary: Carbaryl, a representative of carbamate insecticide, poses potential threat to humans and other non-target organisms as an acetylcholinesterase inhibitor. An Agrobacterium sp. XWY-2 strain capable of utilizing carbaryl as the sole carbon source was isolated, and the carH gene encoding a carbaryl hydrolase was cloned from this strain. The hydrolase, CarH, shows efficient hydrolysis of carbamate pesticides and the key amino acid residues for carbaryl hydrolysis were identified through point mutation experiments.

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2021)

添加到收藏夹

Article Environmental Sciences

Highly specific nanobody against herbicide 2,4-dichlorophenoxyacetic acid for monitoring of its contamination in environmental water

Zhen-Feng Li et al.

Summary: The study successfully developed a nanobody-based immunoassay for monitoring trace levels of 2,4-D in the environment. Utilizing a step-wise strategy from hapten design to nanobody-AP fusion production, the research achieved successful results, demonstrating the method as a convenient and practical tool for monitoring 2,4-D residuals.

SCIENCE OF THE TOTAL ENVIRONMENT (2021)

添加到收藏夹

Article Chemistry, Analytical

A ratiometric fluorescence nanoprobe using CdTe QDs for fast detection of carbaryl insecticide in apple

F. Shahdost-fard et al.

Summary: A novel fluorescence method using cadmium telluride quantum dots was presented for sensitive detection of carbaryl in Iranian apples, with a low detection limit and potential for use in monitoring fruit residues. The enzyme-free visual detection showed satisfactory results in the presence of other pesticides, and was confirmed by high-performance liquid chromatography analysis, suggesting promise for rapid and valid detection of food safety targets.

TALANTA (2021)

添加到收藏夹

Article Environmental Sciences

Study of conformational and functional changes caused by binding of environmental pollutant tonalide to human serum albumin

Hongzhao Xiang et al.

Summary: This study investigated the interaction mechanism between AHTN and HSA, revealing that the binding between AHTN and HSA is a moderate strength spontaneous process driven by hydrophobic forces. AHTN acts as a competitive inhibitor to weaken the esterase-like activity of HSA, potentially impairing the function of HSA.

CHEMOSPHERE (2021)

添加到收藏夹

Article Biochemical Research Methods

Multivalent nanobody as capture antibody-based enzyme linked immunosorbent assay for detection of 3-phenoxybenzoic acid in urine

Jinxin He et al.

Summary: This study demonstrates that assembling single-domain nanobodies into trivalent form can improve the sensitivity and accuracy of ELISA detection, particularly for chemical residues in urine samples.

ANALYTICAL BIOCHEMISTRY (2021)

添加到收藏夹

Article Geochemistry & Geophysics

Effectiveness of Carbaryl, Carbofuran and Metolachlor Retention in Soils under the Influence of Different Colloid

Irmina Cwielag-Piasecka et al.

Summary: The study investigated the affinity of different soil colloids to retain carbaryl, carbofuran, and metolachlor in sandy loam and loam. The results showed that loam soil was a better pesticide retarder than sandy soil, and the addition of humic acid, illite, and goethite increased the sorption of the pesticides significantly. The sorption capacity of the studied pesticides in loamy soil was blocked by natural organic matter potentially due to the formation of organo-mineral complexes.

MINERALS (2021)

添加到收藏夹

Article Chemistry, Analytical

Effect of proteins on the oxidase-like activity of CeO2 nanozymes for immunoassays

Zi-Jian Chen et al.

Summary: This study explores the application of CeO2 nanoparticles in immunoassays and their adsorption behavior towards proteins, demonstrating the potential of CeO2 in bioanalytical chemistry, especially in terms of stability and tolerance to extreme conditions.

ANALYST (2021)

添加到收藏夹

Article Oncology

Identification of anti-CD16a single domain antibodies and their application in bispecific antibodies

Yining Zhao et al.

CANCER BIOLOGY & THERAPY (2020)

添加到收藏夹

Article Environmental Sciences

A rapid and simple fluorescence enzyme-linked immunosorbent assay for tetrabromobisphenol A in soil samples based on a bifunctional fusion protein

Hui-Jun Fu et al.

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2020)

添加到收藏夹

Article Environmental Sciences

High-throughput chemiluminescence immunoassay based on Co2+/hemin synergistic catalysis for sensitive detection tetrabromobisphenol A bis(2-hydroxyethyl) ether in the environments

Lantian Gu et al.

SCIENCE OF THE TOTAL ENVIRONMENT (2020)

添加到收藏夹

Article Biology

Multimeric single-domain antibody complexes protect against bunyavirus infections

Paul J. Wichgers Schreur et al.

ELIFE (2020)

添加到收藏夹

Article Environmental Sciences

Production of a specific monoclonal antibody for 1-naphthol based on novel hapten strategy and development of an easy-to-use ELISA in urine samples

Zi-Jian Chen et al.

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2020)

添加到收藏夹

Review Biochemistry & Molecular Biology

Rapid Multi-Residue Detection Methods for Pesticides and Veterinary Drugs

Min Jia et al.

MOLECULES (2020)

添加到收藏夹

Article Engineering, Environmental

Rapid Airborne Influenza Virus Quantification Using an Antibody-Based Electrochemical Paper Sensor and Electrostatic Particle Concentrator

Jyoti Bhardwaj et al.

ENVIRONMENTAL SCIENCE & TECHNOLOGY (2020)

添加到收藏夹

Article Medicine, Research & Experimental

Biophysical and biochemical characterization of a VHH-based IgG-like bi- and trispecific antibody platform

Lukas Pekar et al.

MABS (2020)

添加到收藏夹

Article Agriculture, Multidisciplinary

Development of an immunoassay for the detection of carbaryl in cereals based on a camelid variable heavy-chain antibody domain

Zhiping Liu et al.

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2019)

添加到收藏夹

Article Biochemical Research Methods

One-step immunoassay for the insecticide carbaryl using a chicken single-chain variable fragment (scFv) fused to alkaline phosphatase

Jinxin He et al.

ANALYTICAL BIOCHEMISTRY (2019)

添加到收藏夹

Article Food Science & Technology

Analysis of Pesticide Residue in Tomatoes by Carbon Nanotubes/β-Cyclodextrin Nanocomposite Reinforced Hollow Fiber Coupled with HPLC

Yawen Ding et al.

JOURNAL OF FOOD SCIENCE (2019)

添加到收藏夹

Article Environmental Sciences

Carbaryl biodegradation by Xylaria sp BNL1 and its metabolic pathway

Fei Li et al.

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2019)

添加到收藏夹

Article Chemistry, Multidisciplinary

Differences between the binding modes of enantiomers S/R-nicotine to acetylcholinesterase

Ji Yang et al.

RSC ADVANCES (2019)

添加到收藏夹

Article Chemistry, Analytical

Fenobody: A Ferritin-Displayed Nanobody with High Apparent Affinity and Half-Life Extension

Kelong Fan et al.

ANALYTICAL CHEMISTRY (2018)

添加到收藏夹

Article Biochemistry & Molecular Biology

Production of Antigen-Binding Fragment against O,O-Diethyl Organophosphorus Pesticides and Molecular Dynamics Simulations of Antibody Recognition

Zi-Jian Chen et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2018)

添加到收藏夹

Article Chemistry, Analytical

A facile indicator box based on Eu3+ functionalized MOF hybrid for the determination of 1-naphthol, a biomarker for carbaryl in urine

Si-Jia Qin et al.

SENSORS AND ACTUATORS B-CHEMICAL (2018)

添加到收藏夹

Article Agriculture, Multidisciplinary

Bispecific Monoclonal Antibody-Based Multianalyte ELISA for Furaltadone Metabolite, Malachite Green, and Leucomalachite Green in Aquatic Products

Feng Wang et al.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2016)

添加到收藏夹

Article Chemistry, Analytical

Multi-analyte enzyme-linked immunosorbent assay for organophosphorus pesticides and neonicotinoid insecticides using a bispecific monoclonal antibody

Xiude Hua et al.

ANALYTICAL METHODS (2013)

添加到收藏夹

Article Chemistry, Analytical

Micellar Liquid Chromatographic Determination of Carbaryl and 1-Naphthol in Water, Soil, and Vegetables

Mei-Liang Chin-Chen et al.

INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY (2012)

添加到收藏夹

Article Chemistry, Applied

Development of chemiluminescence enzyme-linked immunosorbent assay for the screening of metolcarb and carbaryl in orange juice, cabbage and cucumber

J. W. Sun et al.

FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT (2010)

添加到收藏夹

Article Biochemical Research Methods

Gold immunochromatographic assay for simultaneous detection of carbofuran and triazophos in water samples

Yi-Rong Guo et al.

ANALYTICAL BIOCHEMISTRY (2009)

添加到收藏夹

Article Chemistry, Analytical

Development of a flow-through enzyme-linked immunosorbent assay and a dipstick assay for the rapid detection of the insecticide carbaryl

S Wang et al.

ANALYTICA CHIMICA ACTA (2005)

添加到收藏夹

© Peeref 2019-2024. All rights reserved.