Regulation of proline metabolism, AsA-GSH cycle, cadmium uptake and subcellular distribution in Brassica napus L. under the effect of nano-silicon

Cadmium (Cd) is known to have detrimental effects on plant growth and human health. Recent studies showed that silicon nanoparticles (SNPs) can decrease Cd toxicity in plants. Therefore, a study was conducted using 50 & mu;M Cd and 1.50 mM SNPs to investigate Cd uptake, subcellular distribution, proline (Pro) metabolism, and the antioxidant defense system in rapeseed seedlings. In this study, results indicated that Cd stress negatively affected rapeseed growth, and high Cd contents accumulated in both shoots and roots. However, SNPs significantly decreased Cd contents in shoots and roots. Moreover, substantial increases were found in root fresh weight by 40.6% and dry weight by 46.6%, as well as shoot fresh weight by 60.1% and dry weight by 113.7% with the addition of SNPs. Furthermore, the addition of SNPs alleviated oxidative injury by maintaining the ascorbateglutathione (AsA-GSH) cycle and increased Pro biosynthesis which could be due to high activities of & UDelta;1-pyr-roline-5-carboxylate synthase (P5CS) and reductase (P5CR) and decreased proline dehydrogenase (ProDH) activity. Furthermore, the addition of SNPs accumulated Cd in the soluble fraction (42%) and cell wall (45%). Results indicate that SNPs effectively reduce Cd toxicity in rapeseed seedlings which may be effective in promoting both rapeseed productivity and human health preservation.

Automated summary

The study showed that silicon nanoparticles (SNPs) effectively reduced Cd toxicity in rapeseed seedlings by decreasing Cd contents, promoting plant growth, and alleviating oxidative damage. SNPs also influenced the subcellular distribution of Cd and enhanced the synthesis of proline, contributing to the improvement of rapeseed productivity and human health preservation.