4.8 Article

Aberrant DNA N6-methyladenine incorporation via adenylate kinase 1 is suppressed by ADAL deaminase-dependent 2'-deoxynucleotide pool sanitation

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EMBO JOURNAL
卷 42, 期 15, 页码 -

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WILEY
DOI: 10.15252/embj.2023113684

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2'-deoxynucleotide pool sanitation; 6mdA misincorporation; adenylate kinase 1; deaminase ADAL; DNA N-6-methyladenine

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Intracellular decay of N-6-methyladenine (m6A)-containing RNA does not generate free 6mdA species or lead to misincorporation of DNA 6mdA in most tested mammalian cell lines, revealing a sanitation mechanism that prevents 6mdA misincorporation.
Intracellular decay of N-6-methyladenine (m6A)-containing RNA potentially induces aberrant N-6-methyl-2'-adenine (6mdA) misincorporation into DNA. Biophysically, misincorporated 6mdA may destabilize the DNA duplex in a manner similar to bona fide methylated 6mdA DNA, thereby affecting DNA replication and transcription. Utilizing heavy stable isotope labeling and ultrasensitive UHPLC-MS/MS assay, we demonstrate that intracellular m6A-RNA decay does not generate free 6mdA species, nor lead to any misincorporated DNA 6mdA in most mammalian cell lines tested, unveiling the existence of a sanitation mechanism that prevents 6mdA misincorporation. Depletion of deaminase ADAL increases the levels of free 6mdA species, concomitant with the presence of DNA-misincorporated 6mdA resulting from intracellular RNA m6A decay, suggesting that ADAL catabolizes 6mdAMP in vivo. Furthermore, we show that the overexpression of adenylate kinase 1 (AK1) promotes 6mdA misincorporation, while AK1 knockdown diminishes 6mdA incorporation, in ADAL-deficient cells. We conclude that ADAL together with other factors (such as MTH1) contributes to 2'-deoxynucleotide pool sanitation in most cells but compromised sanitation (e.g., in NIH3T3 cells) and increased AK1 expression may facilitate aberrant 6mdA incorporation. This sanitation mechanism may provide a framework for the maintenance of the epigenetic 6mdA landscape.

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